PXD034212

PXD034212 is an original dataset announced via ProteomeXchange.

Title	Impact of neonatal lead exposure on memory: A study highlighting the association between hippocampal synaptic proteome and cognitive decline in rats
Description	Lead (Pb2+) is a heavy metal ubiquitously present in the environment, with unknown essential functions to sustain life. The detrimental effects of Pb2+ on health are known since ancient times, which is also well supported by scientific literature. Incidentally, Pb2+ toxicity is more severe in children than in adults. Various studies highlight the drastic effects of Pb2+ exposure on memory and cognition. However, the exact signalling pathways affected by Pb2+ are still unclear. In the present study, we exposed rat pups to Pb2+ acetate through maternal lactation from day P0 till weaning. The impact of early Pb2+ exposure to spatial cognition was tested using partially baited radial arm maze task three months after weaning. Furthermore, to study the effect of Pb2+ exposure on SUMOylation, the synaptosomal fractions from the hippocampus were purified, and mass spectrometry-based proteomic analysis was performed. Results from behavioral tests revealed that Pb2+ exposure early in life caused severe impairments in spatial cognition with increased reference memory errors and a reduced number of correct choices in PBRAM test. The proteomic analysis revealed differentially altered proteins in Pb2+ exposed rats compared to controls, including SUMO target proteins. Thus, our findings suggest that SUMOylation is a vital protein modification that plays an essential role in memory consolidation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:30:35.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhupesh Mehta
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-30 11:33:01	ID requested
1	2023-03-10 15:01:44	announced
⏵ 2	2023-11-14 07:30:37	announced	2023-11-14: Updated project metadata.

Mohanraj N, Joshi NS, Poulose R, Patil RR, Santhoshkumar R, Kumar A, Waghmare GP, Saha AK, Haider SZ, Markandeya YS, Dey G, Rao LT, Govindaraj P, Mehta B, A proteomic study to unveil lead toxicity-induced memory impairments invoked by synaptic dysregulation. Toxicol Rep, 9():1501-1513(2022) [pubmed]

submitter keyword: lead toxicity, proteomics, hippocampus, memory impairment

Dr. Bhupesh Mehta
contact affiliation	National Institute of Mental Health and Neurosciences
contact email	bhupeshmehta@nimhans.ac.in
lab head
Bhupesh Mehta
contact affiliation	National Institute of Mental Health and Neurosciences
contact email	bhupeshmehta@nimhans.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034212

PRIDE project URI

• PRIDE
  1. PXD034212
     1. Label: PRIDE project
     2. Name: Impact of neonatal lead exposure on memory: A study highlighting the association between hippocampal synaptic proteome and cognitive decline in rats