PXD034179 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pathogen protein modularity enables mimicry of a host phosphatase |
Description | Pathogens rely on a diverse complement of effector proteins to manipulate host cellular processes. The devastating plant pathogen Phytophthora produces numerous effectors that consist of tandem repeats of the “(L)WY” motif. Here, we discovered a specific (L)WY-LWY combination that enables efficient recruitment of the Serine/Threonine protein phosphatase 2A (PP2A) core enzyme in plant hosts. Structural characterization of an effector-PP2A complex and biochemical analyses demonstrate multiple effectors adopt this module at the amino terminus to form functional PP2A holoenzymes but have divergent C-terminal LWY units. Our results discovered a virulence strategy widely employed by Phytophthora in which an essential phosphatase complex of the host is appropriated to promote disease and highlight how diversification in an effector repertoire is promoted by protein modularity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-01 |
AnnouncementXML | Submission_2024-10-01_06:49:48.307.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034179 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jan Sklenar |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-29 13:44:17 | ID requested | |
⏵ 1 | 2024-10-01 06:49:49 | announced | |
Publication List
10.6019/PXD034179; |
Li H, Wang J, Kuan TA, Tang B, Feng L, Wang J, Cheng Z, Sk, ł, enar J, Derbyshire P, Hulin M, Li Y, Zhai Y, Hou Y, Menke FLH, Wang Y, Ma W, Pathogen protein modularity enables elaborate mimicry of a host phosphatase. Cell, 186(15):3196-3207.e17(2023) [pubmed] |
10.1016/j.cell.2023.05.049; |
Keyword List
submitter keyword: Arabidopsis, effector, Phosphatase,Phytophthora |
Contact List
Wenbo Ma |
contact affiliation | The Sainsbury Laboratory, Colney Lane, NR4 7UH, Norwich, Norfolk, United Kingdom |
contact email | Wenbo.Ma@tsl.ac.uk |
lab head | |
Jan Sklenar |
contact affiliation | The Sainsbury Laboratory, Norwich, UK |
contact email | jan.sklenar@tsl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034179
- Label: PRIDE project
- Name: Pathogen protein modularity enables mimicry of a host phosphatase