PXD034179

PXD034179 is an original dataset announced via ProteomeXchange.

Title	Pathogen protein modularity enables mimicry of a host phosphatase
Description	Pathogens rely on a diverse complement of effector proteins to manipulate host cellular processes. The devastating plant pathogen Phytophthora produces numerous effectors that consist of tandem repeats of the “(L)WY” motif. Here, we discovered a specific (L)WY-LWY combination that enables efficient recruitment of the Serine/Threonine protein phosphatase 2A (PP2A) core enzyme in plant hosts. Structural characterization of an effector-PP2A complex and biochemical analyses demonstrate multiple effectors adopt this module at the amino terminus to form functional PP2A holoenzymes but have divergent C-terminal LWY units. Our results discovered a virulence strategy widely employed by Phytophthora in which an essential phosphatase complex of the host is appropriated to promote disease and highlight how diversification in an effector repertoire is promoted by protein modularity.
HostingRepository	PRIDE
AnnounceDate	2024-10-01
AnnouncementXML	Submission_2024-10-01_06:49:48.307.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034179
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Sklenar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-29 13:44:17	ID requested
⏵ 1	2024-10-01 06:49:49	announced

10.6019/PXD034179;

Li H, Wang J, Kuan TA, Tang B, Feng L, Wang J, Cheng Z, Sk, ł, enar J, Derbyshire P, Hulin M, Li Y, Zhai Y, Hou Y, Menke FLH, Wang Y, Ma W, Pathogen protein modularity enables elaborate mimicry of a host phosphatase. Cell, 186(15):3196-3207.e17(2023) [pubmed]

10.1016/j.cell.2023.05.049;

submitter keyword: Arabidopsis, effector, Phosphatase,Phytophthora

Wenbo Ma
contact affiliation	The Sainsbury Laboratory, Colney Lane, NR4 7UH, Norwich, Norfolk, United Kingdom
contact email	Wenbo.Ma@tsl.ac.uk
lab head
Jan Sklenar
contact affiliation	The Sainsbury Laboratory, Norwich, UK
contact email	jan.sklenar@tsl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD034179

PRIDE project URI

• PRIDE
  1. PXD034179
     1. Label: PRIDE project
     2. Name: Pathogen protein modularity enables mimicry of a host phosphatase