PXD034172
PXD034172 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics |
| Description | Protein glycosylation, a complex and heterogeneous post-translational modification that is frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a data-independent acquisition technique for the large-scale mass-spectrometric quantification of glycopeptides in plasma samples. The technique, which we named ‘OxoScan-MS’, identifies oxonium ions as glycopeptide fragments and exploits a sliding-quadrupole dimension to generate comprehensive and untargeted oxonium- ion maps of precursor masses assigned to fragment ions from non-enriched plasma samples. By applying OxoScan-MS to quantify 1,002 glycopeptide features in the plasma glycoproteomes from patients with COVID-19 and healthy controls, we found that severe COVID-19 induces differential glycosylation in IgA, haptoglobin, transferrin and other disease-relevant plasma glycoproteins. OxoScan-MS may allow for the quantitative mapping of glycoproteomes at the scale of hundreds to thousands of samples. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-07-05 |
| AnnouncementXML | Submission_2023-07-05_07:15:38.309.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Matt White |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification |
| Instrument | TripleTOF 6600; Orbitrap Eclipse; ZenoTOF 7600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-05-29 10:57:36 | ID requested | |
| ⏵ 1 | 2023-07-05 07:15:38 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Glycoproteomics, Plasma Biomarkers, Data-independent acquisition |
Contact List
| Prof. Dr. Markus Ralser | |
|---|---|
| contact affiliation | Charite Universitatsmedizin Berlin |
| contact email | markus.ralser@charite.de |
| lab head | |
| Matt White | |
| contact affiliation | Francis Crick Institute |
| contact email | matt.white@crick.ac.uk |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089567/ |
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