PXD034169

PXD034169 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the mechanism of recombinant actifensin and defensin-d2 against multidrug-resistant Pseudomonas aeruginosa and Candida albicans
Description	In this study, we evaluated the protein expression changes in multidrug-resistant P. aeruginosa and C. albicans treated with recombinant defensin-d2 and actifensin from spinach and Actinomyces ruminicola, respectively, for 1 h. The proteome changes in the test organisms were analyzed by LC-MS/MS ESI using label-free data-dependent acquisition (DDA) quantitative proteomics technique. A total of 28 and 9 differentially expressed proteins (DEPs) were identified in the treated P. aeruginosa and C. albicans, respectively, with a 2-fold change threshold and p-value < 0.05. The GO and KEGG enrichment analysis found that DEPs related to binding, catalytic activity, cellular component organization and biogenesis, structural molecule activity and response to stimulus were significantly expressed in actifensin and defensin-treated P. aeruginosa, and the DEPs were mainly involved in DNA replication and repair, translation, membrane transport, energy metabolism, environmental adaptation, and signal transduction. DEPs in actifensin and defensin-treated C. albicans were related to cell growth and death, translation, lipid metabolism and energy metabolism were significantly expressed, and these DEPs mainly involved oxidative phosphorylation, RNA degradation, carbohydrate metabolism, and cell cycle pathways. We also established evidently through the different pathways affected, that the recombinant peptides exerted multiple mechanisms of action against the test organisms, sequentially or simultaneously.
HostingRepository	PRIDE
AnnounceDate	2022-09-17
AnnouncementXML	Submission_2022-09-17_01:08:00.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ifeoluwa Gbala
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-27 06:41:25	ID requested
⏵ 1	2022-09-17 01:08:00	announced

Dataset with its publication pending

submitter keyword: Pseudomonas aeruginosa, Candida albicans, antimicrobial peptides, spinach defensin, actifensin, proteomics, antimicrobial mechanism

IFEOLUWA DEBORAH GBALA
contact affiliation	Molecular Biology and Biotechnology, Pan African University, Institute for Basic Sciences, Technology and Innovation P.O. Box 62000 - 00200 Nairobi, Kenya
contact email	gbalaiffy@gmail.com
lab head
Ifeoluwa Gbala
contact affiliation	Pan African University Institute of Basic Sciences, Technology and Innovation
contact email	gbalaiffy@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034169
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the mechanism of recombinant actifensin and defensin-d2 against multidrug-resistant Pseudomonas aeruginosa and Candida albicans