PXD034136 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Parkin-dependent mitophagy occurs via proteasome-dependent stepwise inwardly directed mitochondrial sub-compartment targeting to lysosomes |
Description | PINK1/parkin-dependent mitophagy initially involves (phospho) ubiquitin-proteasome-dependent degradation of certain outer mitochondrial membrane (OMM) proteins (e.g. mitofusins) and then the recruitment of autophagy effectors to more stable ubiquitinated OMM proteins. It is widely assumed that such ubiquitinated mitochondria are ultimately degraded via wholesale mitophagy. However, we demonstrate that mitochondrial degradation occurs via step-wise delivery of separate mitochondrial sub-compartments to lysosomes. OMM and inner mitochondrial material appears to become separately isolated for autophagolysosomal degradation, not only in parkin-overexpressing HeLa cells but also in cells that express endogenous parkin (human embryonic kidney cells and neural progenitor cells) with slower mitophagy kinetics. The remaining inner mitochondrial material becomes degraded only after much prolonged membrane depolarization, involving another proteasome-sensitive step. These combined microscopy and proteomics analyses lend support to the idea that cell stress-induced parkin-dependent mitophagy is a complex regulated multi-step process with distinct sub-compartments separately targeted to autophagic degradation. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-26 |
AnnouncementXML | Submission_2025-05-25_16:37:11.347.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katharina Zittlau |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-26 01:18:28 | ID requested | |
⏵ 1 | 2025-05-25 16:37:12 | announced | |
Publication List
Lechado-Terradas A, Schepers S, Zittlau KI, Sharma K, Ok O, Fitzgerald JC, Geimer S, Westermann B, Macek B, Kahle PJ, Parkin-dependent mitophagy occurs via proteasome-dependent steps sequentially targeting separate mitochondrial sub-compartments for autophagy. Autophagy Rep, 1(1):576-602(2022) [pubmed] |
10.1080/27694127.2022.2143214; |
Keyword List
submitter keyword: mitochondrial outer membrane |
mitophagy |
parkin |
proteasome |
ubiquitin |
Contact List
Boris Macek |
contact affiliation | Department of Biology, Quantitative Proteomics Group, Interfaculty Institute of Cell Biology, University of Tübingen, Tübingen, Germany |
contact email | boris.macek@uni-tuebingen.de |
lab head | |
Katharina Zittlau |
contact affiliation | University of Tuebingen |
contact email | Katharina.zittlau@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034136
- Label: PRIDE project
- Name: Parkin-dependent mitophagy occurs via proteasome-dependent stepwise inwardly directed mitochondrial sub-compartment targeting to lysosomes