PXD034118

PXD034118 is an original dataset announced via ProteomeXchange.

Title	Combining redox proteomics with proximity labeling to monitor localized cysteine oxidation
Description	Reactive oxygen species (ROS) can modulate protein function through cysteine oxidation. Identifying targets of ROS can provide insight into uncharacterized ROS-regulated pathways. Several redox-proteomic workflows, such as OxICAT, exist to identify sites of cysteine oxidation. However, determining ROS targets localized within subcellular compartments and ROS hotspots remains challenging with existing workflows. Here, we present a chemoproteomic platform, PL-OxICAT, which combines proximity labeling (PL) with OxICAT to monitor localized cysteine oxidation events. We show that TurboID-based PL-OxICAT can monitor cysteine oxidation events within subcellular compartments such as the mitochondrial matrix and intermembrane space. Furthermore, we use APEX-based PL-OxICAT to monitor oxidation events within ROS hotspots by using endogenous ROS as the source of peroxide for APEX activation. Together, these platforms further hone our ability to monitor cysteine oxidation events within specific subcellular locations and ROS hotspots and provide a deeper understanding of the protein targets of endogenous and exogenous ROS.
HostingRepository	PRIDE
AnnounceDate	2024-05-21
AnnouncementXML	Submission_2024-05-21_09:34:16.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eleni Kisty
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 06:54:15	ID requested
⏵ 1	2024-05-21 09:34:17	announced

Kisty EA, Falco JA, Weerapana E, Redox proteomics combined with proximity labeling enables monitoring of localized cysteine oxidation in cells. Cell Chem Biol, 30(3):321-336.e6(2023) [pubmed]

10.1016/j.chembiol.2023.02.006;

submitter keyword: Cysteine oxidation, APEX, reactive oxygen species, OxICAT, TurboID, proximity labeling

Eranthie Weerapana
contact affiliation	Chemistry Department, Boston College, USA
contact email	eranthie@bc.edu
lab head
Eleni Kisty
contact affiliation	Boston College
contact email	kisty@bc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034118
     1. Label: PRIDE project
     2. Name: Combining redox proteomics with proximity labeling to monitor localized cysteine oxidation