PXD034115 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling |
Description | NIMA-related kinase 9 (NEK9) was identified to interact with the essential myosin light chain (ELC). To validate these findings ascorbate peroxidase (APEX) catalyzed proximity labeling (Hung et al. Nat Protoc. 2016) was performed. To do so, human ELC fused to APEX was overexpressed in HEK293 cells. Empty pcDNA3-APEX2-NES vector serves as negative control. Half of each sample was treated with H2O2, which catalyzes protein labeling in close proximity of ELC. After streptavidin pull down of labeled proteins, the samples were analyzed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-24 |
AnnouncementXML | Submission_2022-07-24_02:37:11.202.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Ruppert |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-25 06:52:39 | ID requested | |
⏵ 1 | 2022-07-24 02:37:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: BioID, ELC, NEK9 |
Contact List
Thomas Ruppert |
contact affiliation | ZMBH, University Heidelberg Im Neuenheimer Feld 282 69120 Heidelbe4rg Germany |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
lab head | |
Thomas Ruppert |
contact affiliation | ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034115 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034115
- Label: PRIDE project
- Name: Analysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling