PXD034115

PXD034115 is an original dataset announced via ProteomeXchange.

Title	Analysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling
Description	NIMA-related kinase 9 (NEK9) was identified to interact with the essential myosin light chain (ELC). To validate these findings ascorbate peroxidase (APEX) catalyzed proximity labeling (Hung et al. Nat Protoc. 2016) was performed. To do so, human ELC fused to APEX was overexpressed in HEK293 cells. Empty pcDNA3-APEX2-NES vector serves as negative control. Half of each sample was treated with H2O2, which catalyzes protein labeling in close proximity of ELC. After streptavidin pull down of labeled proteins, the samples were analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2022-07-24
AnnouncementXML	Submission_2022-07-24_02:37:11.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 06:52:39	ID requested
⏵ 1	2022-07-24 02:37:11	announced

Dataset with its publication pending

submitter keyword: BioID, ELC, NEK9

Thomas Ruppert
contact affiliation	ZMBH, University Heidelberg Im Neuenheimer Feld 282 69120 Heidelbe4rg Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034115

PRIDE project URI

• PRIDE
  1. PXD034115
     1. Label: PRIDE project
     2. Name: Analysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling