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PXD034115

PXD034115 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling
DescriptionNIMA-related kinase 9 (NEK9) was identified to interact with the essential myosin light chain (ELC). To validate these findings ascorbate peroxidase (APEX) catalyzed proximity labeling (Hung et al. Nat Protoc. 2016) was performed. To do so, human ELC fused to APEX was overexpressed in HEK293 cells. Empty pcDNA3-APEX2-NES vector serves as negative control. Half of each sample was treated with H2O2, which catalyzes protein labeling in close proximity of ELC. After streptavidin pull down of labeled proteins, the samples were analyzed by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2022-07-24
AnnouncementXMLSubmission_2022-07-24_02:37:11.202.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Ruppert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-25 06:52:39ID requested
12022-07-24 02:37:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: BioID, ELC, NEK9
Contact List
Thomas Ruppert
contact affiliationZMBH, University Heidelberg Im Neuenheimer Feld 282 69120 Heidelbe4rg Germany
contact emailt.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliationZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact emailt.ruppert@zmbh.uni-heidelberg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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