PXD034101

PXD034101 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-Cov-2 innate immune evasion
Description	We used two model systems to characterise the changes caused by SARS-CoV-2 using quantitative proteomics. We used primary human epithelial cells differentiated at the air-liquid interface, first describing the relative abundance of proteins found in each major cell type of the epithelium, and then characterised the infection of ciliated cells specifically as these showed the greatest susceptibility to SARS-CoV-2 infection. We also used a lung epithelial cell line, CaLu-3, and compared the infection with B.29 and B.1.1.7 (Alpha) variant over a time-course of infection. In each case, immunostaining for nucleoprotein combined with FACS allowed for the purification of infected cells from uninfected ‘bystander’ cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:41:33.090.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034101
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Williamson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 03:18:30	ID requested
1	2022-11-30 03:03:58	announced
2	2023-01-25 13:10:11	announced	2023-01-25: Updated project metadata.
⏵ 3	2023-11-14 07:41:33	announced	2023-11-14: Updated project metadata.

10.6019/PXD034101;

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: CALU,SARS CoV-2, HBEC, Proteomics

Paul Lehner
contact affiliation	Cambridge Institute of Therapeutic Immunology and Infectious Disease Jeffrey Cheah Biomedical Centre Cambridge Biomedical Campus Puddicombe Way Cambridge CB2 OAW
contact email	pjl30@cam.ac.uk
lab head
James Williamson
contact affiliation	University of Cambridge
contact email	jcw76@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD034101

PRIDE project URI

• PRIDE
  1. PXD034101
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of SARS-CoV-2 infection of primary human airway ciliated cells and lung epithelial cells demonstrates the effectiveness of SARS-Cov-2 innate immune evasion