PXD034099

PXD034099 is an original dataset announced via ProteomeXchange.

Title	Rumen microbiome and effect of monensin
Description	The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigned to fibrolytic bacteria and glycoside hydrolases, sugar transporters and carbohydrate metabolism. Reduced amounts of ammonium as well as branched-chain fatty acids pointed towards a decreased proteolytic activity. The family Prevotellaceae exhibited increased resilience in the presence of monensin, with a switch of metabolism from acetate to succinate production. Prevotella species harbor a membrane bound electron transfer complex, which drives the reduction of fumarate to succinate, the substrate for propionate production in the rumen habitat. Besides the increased succinate production, a concomitant depletion of methane concentration was observed upon monensin exposure. Our study demonstrates that Prevotella sp. shifts its metabolism successfully in response to monensin exposure and Prevotellaceae represents the key bacterial family stabilizing the rumen microbiota during exposure to monensin.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:29:33.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Seifert
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 03:10:53	ID requested
1	2022-12-11 11:31:08	announced
⏵ 2	2023-11-14 07:29:34	announced	2023-11-14: Updated project metadata.

Trautmann A, Schleicher L, Koch A, G, ü, nther J, Steuber J, Seifert J, A shift towards succinate-producing Prevotella in the ruminal microbiome challenged with monensin. Proteomics, 23(21-22):e2200121(2023) [pubmed]

submitter keyword: Prevotella, microbiome, monensin,rumen

Jana Seifert
contact affiliation	Institute of Animal Science, University of Hohenheim, Stuttgart, Germany
contact email	jseifert@uni-hohenheim.de
lab head
Jana Seifert
contact affiliation	University of Hohenheim
contact email	jseifert@uni-hohenheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD034099

PRIDE project URI

• PRIDE
  1. PXD034099
     1. Label: PRIDE project
     2. Name: Rumen microbiome and effect of monensin