PXD034091 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fibroblast-tracks are topographical cues that direct cancer cell migration |
Description | Fibroblasts rely on adhesive structures to migrate. It is generally thought that adhesive structures disassemble once at the rear of the cell to release the cell and allow further movement. However, a significant portion of adhesions is not disassembled and is instead left on the substrate. We observed that virtually all the non-resorbed adhesions remain connected to the substrate by the means of integrin β5. Forward cell migration results in plasma membrane pulling giving rise to tubular structures that are then left on the ground, forming a network of lipid tracks that persist for several days, thus altering substrate’s topography. Cancer cells of different origin exploit fibroblast-tracks as migration railways. The adhesion of cancer cells along tracks is not mediated by focal adhesions, but rather by frustrated clathrin-coated structures (CCSs). Analysis of the composition of track generated by cancer associated fibroblasts allowed to identify integrin αv as the receptor on cancer cells responsible for track recognition. Some cancer cell lines are not able to perform durotaxis (rigidity driven migration), while fibroblasts are very good at it. We thus tested whether, in the presence of fibroblast-tracks, non-durotactic cancer cells would become durotactic by following tracks and this was indeed the case. Hence, fibroblast-tracks are a novel structure capable of physically directing cell migration of different cell types. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:03:41.878.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vanessa Masson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-25 03:07:28 | ID requested | |
1 | 2023-09-22 08:29:36 | announced | |
2 | 2023-11-14 09:05:20 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:03:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1126/sciadv.ade2120; |
Baschieri F, Illand A, Barbazan J, Zajac O, Henon C, Loew D, Dingli F, Vignjevic DM, L, é, v, ê, que-Fort S, Montagnac G, Fibroblasts generate topographical cues that steer cancer cell migration. Sci Adv, 9(33):eade2120(2023) [pubmed] |
Keyword List
submitter keyword: cancer,CAFs, extracellular matrix |
Contact List
Damarys Loew |
contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
contact email | damarys.loew@curie.fr |
lab head | |
Vanessa Masson |
contact affiliation | Institut Curie - Centre de Recherche |
contact email | vanessa.masson@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD034091 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034091
- Label: PRIDE project
- Name: Fibroblast-tracks are topographical cues that direct cancer cell migration