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PXD034091

PXD034091 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFibroblast-tracks are topographical cues that direct cancer cell migration
DescriptionFibroblasts rely on adhesive structures to migrate. It is generally thought that adhesive structures disassemble once at the rear of the cell to release the cell and allow further movement. However, a significant portion of adhesions is not disassembled and is instead left on the substrate. We observed that virtually all the non-resorbed adhesions remain connected to the substrate by the means of integrin β5. Forward cell migration results in plasma membrane pulling giving rise to tubular structures that are then left on the ground, forming a network of lipid tracks that persist for several days, thus altering substrate’s topography. Cancer cells of different origin exploit fibroblast-tracks as migration railways. The adhesion of cancer cells along tracks is not mediated by focal adhesions, but rather by frustrated clathrin-coated structures (CCSs). Analysis of the composition of track generated by cancer associated fibroblasts allowed to identify integrin αv as the receptor on cancer cells responsible for track recognition. Some cancer cell lines are not able to perform durotaxis (rigidity driven migration), while fibroblasts are very good at it. We thus tested whether, in the presence of fibroblast-tracks, non-durotactic cancer cells would become durotactic by following tracks and this was indeed the case. Hence, fibroblast-tracks are a novel structure capable of physically directing cell migration of different cell types.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:03:41.878.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVanessa Masson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-25 03:07:28ID requested
12023-09-22 08:29:36announced
22023-11-14 09:05:20announced2023-11-14: Updated project metadata.
32024-10-22 06:03:42announced2024-10-22: Updated project metadata.
Publication List
10.1126/sciadv.ade2120;
Baschieri F, Illand A, Barbazan J, Zajac O, Henon C, Loew D, Dingli F, Vignjevic DM, L, é, v, ê, que-Fort S, Montagnac G, Fibroblasts generate topographical cues that steer cancer cell migration. Sci Adv, 9(33):eade2120(2023) [pubmed]
Keyword List
submitter keyword: cancer,CAFs, extracellular matrix
Contact List
Damarys Loew
contact affiliationHead of the Curie Institute Mass Spectrometry Platform
contact emaildamarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliationInstitut Curie - Centre de Recherche
contact emailvanessa.masson@curie.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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