PXD034089

PXD034089 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis Circadian Clock RVE8 LNK1 LNK2 Interactome LC-MS
Description	The timing of many molecular and physiological processes in plants occurs at a specific time of day. The circadian MYB-like transcription factor REVEILLE 8 (RVE8) interacts with its transcriptional coactivators NIGHT LIGHT INDUCIBLE AND CLOCK REGULATED 1 (LNK1) and LNK2 to promote the expression of evening-phased clock genes and cold tolerance factors. While genetic approaches have commonly been used to discover new connections within the clock and between other pathways, here we use affinity purification coupled with mass spectrometry to discover time-of-day-specific protein interactors of the RVE8-LNK1/2 complex. Our clock proteins are tagged with a 6X-His-3X-FLAG C-terminal (HFC) affinity tag and purified through anti-FLAG immunoprecipitation and His-tag isolation affinity purification. We also performed affinity purification-mass spectrometry of 35S::YFP-COR27 and 35S::GFP-COR28, which were immunoprecipitated with anti-GFP TRAP beads. Experiment was performed on 10-day-old seedlings grown under 12 hours light: 12 hours dark 22 degrees Celsius.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:55.908.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034089
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dmitri Nusinow
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 02:53:57	ID requested
1	2023-04-24 15:11:01	announced
⏵ 2	2023-11-14 08:45:04	announced	2023-11-14: Updated project metadata.

Sorkin ML, Tzeng SC, King S, Romanowski A, Kahle N, Bindbeutel R, Hiltbrunner A, Yanovsky MJ, Evans BS, Nusinow DA, COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex. Plant Physiol, 192(3):2436-2456(2023) [pubmed]

10.6019/PXD034089;

submitter keyword: Arabidopsis thaliana, affinity purification, circadian clock, LCMS

Dmitri Nusinow
contact affiliation	Donald Danforth Plant Science Center, St. Louis, MO
contact email	dnusinow@danforthcenter.org
lab head
Dmitri Nusinow
contact affiliation	Donald Danforth Plant Science Center
contact email	dnusinow@danforthcenter.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD034089

PRIDE project URI

• PRIDE
  1. PXD034089
     1. Label: PRIDE project
     2. Name: Arabidopsis Circadian Clock RVE8 LNK1 LNK2 Interactome LC-MS