PXD034087

PXD034087 is an original dataset announced via ProteomeXchange.

Title	Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
Description	In this study, we developed a model system for the assessment of small molecule-protein interactions using intact cells treated with a commercially available highly specific mitogen-activated protein kinase (MEK) 1/2 inhibitor to prepare a set of unfractionated test samples labeled with tandem mass tags. Our objective was to improve qualitative and quantitative aspects of MS-TSA as well as its efficiency and accuracy. We evaluated individually and for the first time in combination ΦSDM, FAIMS, and SIILCC as an improved MS-based acquisition approach for thermal stability assays (iMAATSA). PSM-level filtering was preliminarily investigated as an approach to reduce melting curve variation and improve the accuracy of Tm measurements.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:14.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clifford Phaneuf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 02:39:35	ID requested
1	2023-05-10 10:39:23	announced
⏵ 2	2023-11-14 08:46:23	announced	2023-11-14: Updated project metadata.

Phaneuf CG, Aizikov K, Grinfeld D, Kreutzmann A, Mourad D, Lange O, Dai D, Zhang B, Belenky A, Makarov AA, Ivanov AR, Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays. Commun Chem, 6(1):64(2023) [pubmed]

submitter keyword: CETSA, mass spectrometry, TPP, target identification, thermal stability assays, drug-protein interactions

Prof. Alexander Ivanov
contact affiliation	Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States
contact email	a.ivanov@northeastern.edu
lab head
Clifford Phaneuf
contact affiliation	sanofi
contact email	clifford.phaneuf@sanofi.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD034087

PRIDE project URI

• PRIDE
  1. PXD034087
     1. Label: PRIDE project
     2. Name: Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays