PXD034086

PXD034086 is an original dataset announced via ProteomeXchange.

Title	Structural- and Site-specific N-glycosylation Characterization of COVID-19 Virus Spike with StrucGP
Description	Spike (S) protein plays a key role in COVID-19 (SARS-CoV-2) infection and host-cell entry. Previous studies have systematically analyzed site-specific glycans compositions of S protein. Here, we further provide structure-clear N-glycosylation of S protein at site-specific level by using our recently developed structure- and site-specific N-glycoproteomics sequencing algorithm StrucGP. In addition to the common N-glycans as detected in previous studies, many uncommon glycosylation structures such as LacdiNAc structures, Lewis structures, Mannose 6-Phosphate (M6P) residues and bisected core structures were unambiguously mapped at a total of 20 glycosites in the S protein trimer and protomer. These data further supports the glycosylation structural-functional investigations of COVID-19 virus Spike.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:20:31.266.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bojing Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 02:39:10	ID requested
1	2022-10-13 04:17:44	announced
⏵ 2	2023-11-14 07:20:33	announced	2023-11-14: Updated project metadata.

Zhu B, Chen Z, Shen J, Xu Y, Lan R, Sun S, Glycosylation Characterization of COVID-19 Virus Spike with StrucGP. Anal Chem, 94(36):12274-12279(2022) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: COVID-19, LC-MSMS, StrucGP, Spike, Glycopeptides

Shisheng Sun
contact affiliation	College of Life Sciences, Northwest University, Xi’an 710069, China
contact email	suns@nwu.edu.cn
lab head
Bojing Zhu
contact affiliation	Northwest University
contact email	bojingzhu@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034086

PRIDE project URI

• PRIDE
  1. PXD034086
     1. Label: PRIDE project
     2. Name: Structural- and Site-specific N-glycosylation Characterization of COVID-19 Virus Spike with StrucGP