PXD034064

PXD034064 is an original dataset announced via ProteomeXchange.

Title	Total synthesis of rosette inhibitor IOR allows its functional analysis and evaluation of sulfonolipid-based chemical probes
Description	The predatory choanoflagellate Salpingoeca rosetta has emerged as important model systems to study the evolution of multicellularity and chemical origin of bacterial-eukaryote communication mechanisms. In this study we elaborated on the function and possible proteogenic binding partners of the bacterial sulfonolipid IOR-1A, which serves as bacterial inhibitor of rosette formation in S. rosetta. To study the function of IOR-1A, a new, modular and scalable total synthesis of IOR-1A (six steps, 30% overall yield) was developed, which features a decarboxylative cross-coupling reaction of a desymmetrized tartaric acid derivative carrying a protected sulfonic acid moiety with an alkyl zink reagents of choice. Synthesis of twelve IOR derivates, including fluorescent and photoaffinity-based probes, allowed to determine the abundance of IOR-1A, evaluation of structure-activity relations, localization studies within S. rosetta and de novo chemical proteomic identification of IOR-binding proteins in bacteria and S. rosetta. The combined results will catalyse future studies aiming at deciphering the biochemical basis of cell differentiation processes within rosette formation of S. rosetta.
HostingRepository	PRIDE
AnnounceDate	2022-08-29
AnnouncementXML	Submission_2022-08-29_10:53:51.191.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Algoriphagus machipongonensis; NCBI TaxID: 388413; scientific name: Salpingoeca rosetta; NCBI TaxID: 946362; scientific name: Echinicola pacifica; NCBI TaxID: 346377;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 01:43:21	ID requested
⏵ 1	2022-08-29 10:53:52	announced

Ragu, ž L, Peng CC, Rutaganira FUN, Kr, ü, ger T, Stani, š, i, ć A, Jautzus T, Kries H, Kniemeyer O, Brakhage AA, King N, Beemelmanns C, Total Synthesis and Functional Evaluation of IORs, Sulfonolipid-based Inhibitors of Cell Differentiation in Salpingoeca rosetta. Angew Chem Int Ed Engl, 61(41):e202209105(2022) [pubmed]

submitter keyword: choanoflagellate, Salpingoeca rosetta, sulfonosphingolipids, photoaffinity-labeling, LC-MS/MS

Axel A. Brakhage
contact affiliation	Department of Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena (Leibniz-HKI) Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034064
     1. Label: PRIDE project
     2. Name: Total synthesis of rosette inhibitor IOR allows its functional analysis and evaluation of sulfonolipid-based chemical probes