PXD034051

PXD034051 is an original dataset announced via ProteomeXchange.

Title	p38a regulated networks in cancer cell homeostasis
Description	p38a (encoded by MAPK14) is a protein kinase that regulates cellular responses to almost all types of environmental and intracellular stresses. Upon activation, p38a phosphorylates various substrates both in the cytoplasm and nucleus, allowing this pathway to regulate a wide variety of cellular processes, including cell proliferation, differentiation or survival. To investigate the signaling networks regulated by p38a during cancer cell homeostasis, we performed proteomic and phosphoproteomic analysis. Our study identified with high confidence 35 proteins and 114 phosphosites that are modulated by p38a, and highlighted the implication of other protein kinases, such as MK2 and mTOR, in p38a-regulated cellular processes. Moreover, functional analysis of the proteome and phosphoproteome data revealed an important contribution of p38a to the regulation of the processes of cell adhesion, DNA replication and RNA metabolism. We provide experimental evidence supporting that p38a negatively regulates cell-cell adhesion, and showed that this p38a function is likely mediated by the transcriptional modulation of the adaptor protein ArgBP2. Collectively, our results illustrate the complexity of the p38a regulated signaling networks, provide valuable information on p38a-dependent phosphorylation events in cancer cell homeostasis, and document a mechanism by which p38a regulates the adhesion capacity of cancer cells.
HostingRepository	PRIDE
AnnounceDate	2023-02-10
AnnouncementXML	Submission_2023-02-10_06:53:32.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarinaGay
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-25 01:01:33	ID requested
⏵ 1	2023-02-10 06:53:33	announced

Dataset with its publication pending

submitter keyword: phosphoproteome, ArgBP2, cancer cell homeostasis, cell adhesion,p38a

Angel R.Nebreda
contact affiliation	Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain. ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain.
contact email	angel.nebreda@irbbarcelona.org
lab head
MarinaGay
contact affiliation	Institute for Research in Biomedicine
contact email	marina.gay@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD034051

PRIDE project URI

• PRIDE
  1. PXD034051
     1. Label: PRIDE project
     2. Name: p38a regulated networks in cancer cell homeostasis