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PXD034039

PXD034039 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6
DescriptionThe kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed chemical crosslinking mass spectrometry of the Trypanosoma brucei KKT2 DPB/KKT1C complex and KKT1/KKT6 complex. We also performed immunoprecipitation and mass spectrometry of KKT1 fragments (KKT1N and KKT1C) that were ectopically expressed and purified from trypanosomes.
HostingRepositoryPRIDE
AnnounceDate2022-06-10
AnnouncementXMLSubmission_2022-06-10_08:22:51.469.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBungo Akiyoshi
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-24 08:28:27ID requested
12022-06-10 08:22:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Trypanosoma brucei, kinetoplastid kinetochore, KKT1, KKT2, KKT6
Contact List
Bungo Akiyoshi
contact affiliationDepartment of Biochemistry, University of Oxford
contact emailbungo.akiyoshi@bioch.ox.ac.uk
lab head
Bungo Akiyoshi
contact affiliationUniversity of Oxford
contact emailbungo.akiyoshi@bioch.ox.ac.uk
dataset submitter
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