PXD034039 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6 |
Description | The kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed chemical crosslinking mass spectrometry of the Trypanosoma brucei KKT2 DPB/KKT1C complex and KKT1/KKT6 complex. We also performed immunoprecipitation and mass spectrometry of KKT1 fragments (KKT1N and KKT1C) that were ectopically expressed and purified from trypanosomes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-10 |
AnnouncementXML | Submission_2022-06-10_08:22:51.469.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bungo Akiyoshi |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-24 08:28:27 | ID requested | |
⏵ 1 | 2022-06-10 08:22:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Trypanosoma brucei, kinetoplastid kinetochore, KKT1, KKT2, KKT6 |
Contact List
Bungo Akiyoshi |
contact affiliation | Department of Biochemistry, University of Oxford |
contact email | bungo.akiyoshi@bioch.ox.ac.uk |
lab head | |
Bungo Akiyoshi |
contact affiliation | University of Oxford |
contact email | bungo.akiyoshi@bioch.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034039
- Label: PRIDE project
- Name: Mass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6