PXD034036

PXD034036 is an original dataset announced via ProteomeXchange.

Title	Proteome and secretory proteome of UCA-PSCs and WJ-MSCs
Description	A total of 1× 107 cultured UCA-PSCs and WJ-MSCs at P4 were lysed with 300 μL of SDS lysis buffer containing protease inhibitor and 1 μM PMSF in 1.5-mL tubes. Each sample was ultrasonicated on ice for 3 min. After centrifugation twice at 2000 ×g for 10 min at 4°C, the supernatant was collected for total protein measurement and further LC-MS analysis. Cell supernatants (5 mL/106 cells) of P4 UCA-PSCs and WJ-MSCs cultured in basic DMEM without serum or antibiotics for 48 h were collected. After concentration in 3-KD ultrafiltration centrifuge tubes, the cell supernatants were frozen and resuspended in SDS solution. After centrifugation, the supernatants from UCA-PSC and WJ-MSC cultures were obtained.Secretory proteome of UCA-PSCs and WJ-MSCs was further analyzed.
HostingRepository	iProX
AnnounceDate	2022-05-24
AnnouncementXML	Submission_2022-05-24_05:49:00.223.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lijun Ding
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-24 05:48:49	ID requested
⏵ 1	2022-05-24 05:49:00	announced

Dataset with its publication pending

submitter keyword: UCA-PSCs, WJ-MSCs, proteome, secretory proteome

Haixiang Sun
contact affiliation	Nanjing Drum Tower Hospital, Nanjing University Medical School
contact email	stevensunz@163.com
lab head
Lijun Ding
contact affiliation	Nanjing Drum Tower Hospital, Nanjing University Medical School
contact email	xmljding@163.com
dataset submitter

iProX dataset URI