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PXD034036

PXD034036 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome and secretory proteome of UCA-PSCs and WJ-MSCs
DescriptionA total of 1× 107 cultured UCA-PSCs and WJ-MSCs at P4 were lysed with 300 μL of SDS lysis buffer containing protease inhibitor and 1 μM PMSF in 1.5-mL tubes. Each sample was ultrasonicated on ice for 3 min. After centrifugation twice at 2000 ×g for 10 min at 4°C, the supernatant was collected for total protein measurement and further LC-MS analysis. Cell supernatants (5 mL/106 cells) of P4 UCA-PSCs and WJ-MSCs cultured in basic DMEM without serum or antibiotics for 48 h were collected. After concentration in 3-KD ultrafiltration centrifuge tubes, the cell supernatants were frozen and resuspended in SDS solution. After centrifugation, the supernatants from UCA-PSC and WJ-MSC cultures were obtained.Secretory proteome of UCA-PSCs and WJ-MSCs was further analyzed.
HostingRepositoryiProX
AnnounceDate2022-05-24
AnnouncementXMLSubmission_2022-05-24_05:49:00.223.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLijun Ding
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-24 05:48:49ID requested
12022-05-24 05:49:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: UCA-PSCs, WJ-MSCs, proteome, secretory proteome
Contact List
Haixiang Sun
contact affiliationNanjing Drum Tower Hospital, Nanjing University Medical School
contact emailstevensunz@163.com
lab head
Lijun Ding
contact affiliationNanjing Drum Tower Hospital, Nanjing University Medical School
contact emailxmljding@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI