PXD034036
PXD034036 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome and secretory proteome of UCA-PSCs and WJ-MSCs |
Description | A total of 1× 107 cultured UCA-PSCs and WJ-MSCs at P4 were lysed with 300 μL of SDS lysis buffer containing protease inhibitor and 1 μM PMSF in 1.5-mL tubes. Each sample was ultrasonicated on ice for 3 min. After centrifugation twice at 2000 ×g for 10 min at 4°C, the supernatant was collected for total protein measurement and further LC-MS analysis. Cell supernatants (5 mL/106 cells) of P4 UCA-PSCs and WJ-MSCs cultured in basic DMEM without serum or antibiotics for 48 h were collected. After concentration in 3-KD ultrafiltration centrifuge tubes, the cell supernatants were frozen and resuspended in SDS solution. After centrifugation, the supernatants from UCA-PSC and WJ-MSC cultures were obtained.Secretory proteome of UCA-PSCs and WJ-MSCs was further analyzed. |
HostingRepository | iProX |
AnnounceDate | 2022-05-24 |
AnnouncementXML | Submission_2022-05-24_05:49:00.223.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lijun Ding |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-05-24 05:48:49 | ID requested | |
⏵ 1 | 2022-05-24 05:49:00 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: UCA-PSCs, WJ-MSCs, proteome, secretory proteome |
Contact List
Haixiang Sun | |
---|---|
contact affiliation | Nanjing Drum Tower Hospital, Nanjing University Medical School |
contact email | stevensunz@163.com |
lab head | |
Lijun Ding | |
contact affiliation | Nanjing Drum Tower Hospital, Nanjing University Medical School |
contact email | xmljding@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |