PXD034034

PXD034034 is an original dataset announced via ProteomeXchange.

Title	Human Proteome of Colorectal Cancer by High Performance Liquid Chromatography-Mass Spectrometry
Description	In order to explore the proteomic characteristics of colorectal cancer, we collected cancer tissues and adjacent normal tissues of postoperative colorectal cancer patients for proteomic detection, based on ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) and data-independent acquisition mode (DIA). All samples were processed by DIA individually to assess the proteome differences. MS1 and MS2 data were all acquired, and samples acquisition by random order. The iRT kit (Ki3002, Biognosys AG, Switzerland) was added to all of the samples to calibrate the retention time of extracted peptide peaks. The statistical analysis of the DIA dataset was performed by Spectronaut 15(Biognosys AG, Switzerland) including data normalization and relative protein quantification. After t-Test and corrected by Benjaminiand Hochberg algorithm, different expressed proteins were filtered.
HostingRepository	iProX
AnnounceDate	2022-05-23
AnnouncementXML	Submission_2023-01-03_01:45:21.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qianqian Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-23 18:23:22	ID requested
⏵ 1	2023-01-03 01:45:21	announced

Dataset with its publication pending

submitter keyword: Colorectal cancer, Proteome, Mass Spectrometry

Shukun Yao
contact affiliation	China-Japan Friendship Hospital
contact email	shukunyao@126.com
lab head
Qianqian Wang
contact affiliation	Peking University China-Japan Friendship School of Clinical Medicine
contact email	604749831@qq.com
dataset submitter

iProX dataset URI