PXD034024

PXD034024 is an original dataset announced via ProteomeXchange.

Title	Substrate trapping approach identifies TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes
Description	The tripartite motif (TRIM) family of E3 ubiquitin ligases is well known for its roles in antiviral restriction and innate immunity regulation, in addition to many other cellular pathways. In particular, TRIM25-mediated ubiquitination affects both carcinogenesis and antiviral response. While individual substrates have been identified for TRIM25, it remains unclear how it regulates diverse processes. Here we characterized a mutation, R54P, critical for TRIM25 catalytic activity, which we successfully utilized to “trap” substrates. We demonstrated that TRIM25 targets proteins implicated in stress granule formation (G3BP1/2), nonsense-mediated mRNA decay (UPF1), and nucleoside synthesis (NME1). R54P abolishes TRIM25 inhibition of alphaviruses independently of the host interferon response, suggesting that this antiviral effect is a direct consequence of ubiquitination. Consistent with that, we observed diminished antiviral activity upon knockdown of several TRIM25-R54P specific interactors including NME1. Our findings highlight that multiple substrates mediate the cellular and antiviral activities of TRIM25, illustrating the multi-faceted role of this ubiquitination network in diverse biological processes.
HostingRepository	PRIDE
AnnounceDate	2022-08-02
AnnouncementXML	Submission_2022-08-01_19:59:49.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yasaman Jami-Alahmadi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-23 03:27:51	ID requested
⏵ 1	2022-08-01 19:59:49	announced

Dataset with its publication pending

submitter keyword: TRIM25, ubiquitination,lcms

Melody Li
contact affiliation	UCLA
contact email	ManHingLi@mednet.ucla.edu
lab head
Yasaman Jami-Alahmadi
contact affiliation	UCLA
contact email	yjami@ucla.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034024
     1. Label: PRIDE project
     2. Name: Substrate trapping approach identifies TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes