PXD034012 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics investigation of Nucleostemin role in Nucleo-phosmin mutated OCI-AML 3 cell line |
Description | Nucleostemin (NS; product of the GNL3 gene) is a nucleolar/nucleoplasm shuttling GTPase whose levels are high in stem cells while rapidly decreasing upon differentiation. NS levels are also high in several solid and hematological neoplasms, including acute myeloid leukemia (AML). While a role in telomere maintenance, response to stress stimuli and in favoring DNA repair has been proposed in solid cancers, little or no information is available as to the role of nucleostemin in AML. Here we investigate this issue with a proteomics approach. We use as a model system the OCI-AML 3 cell line harboring a heterozygous mutation at the NPM1 gene, which is the most frequent driver mutation in AML (approximately 30% of total AML cases). We show that NS is highly expressed in this cell line but, contrary to what previously shown in other cancers, its presence is dispensable for cell growth and viability. However, proteomics analysis of OCI-AML 3 cell line before and after nu-cleostemin (NS) silencing showed several effects in different biological functions as highlighted by ingenuity pathway analysis (IPA). In particular, we report an effect in down-regulating DNA repair through homologous recombination and we confirmed higher DNA damage rate in OCI-AML 3 cells when NS is depleted, which considerably increases upon stress induced by the topoisomerase II inhibitor etoposide. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-11 |
AnnouncementXML | Submission_2022-08-11_04:48:35.923.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maria Concetta Cufaro |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-20 10:42:13 | ID requested | |
⏵ 1 | 2022-08-11 04:48:36 | announced | |
Publication List
Cela I, Cufaro MC, Fucito M, Pieragostino D, Lanuti P, Sallese M, Del Boccio P, Di Matteo A, Allocati N, De Laurenzi V, Federici L, Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line. Int J Mol Sci, 23(14):(2022) [pubmed] |
Keyword List
submitter keyword: shotgun proteomics, Nucleophosmin, Acute Myeloid Leukemia, Nucleostemin |
Contact List
Piero Del Boccio |
contact affiliation | Department of Pharmacy, Univeristy of Chieti, Italy |
contact email | piero.delboccio@unich.it |
lab head | |
Maria Concetta Cufaro |
contact affiliation | University "G. d'Annunzio" of Chieti |
contact email | maria.cufaro@unich.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034012
- Label: PRIDE project
- Name: Proteomics investigation of Nucleostemin role in Nucleo-phosmin mutated OCI-AML 3 cell line