PXD034000

PXD034000 is an original dataset announced via ProteomeXchange.

Title	Mechanisms associated with systemic effects of cancer
Description	Cancer is considered as a disease of a specific organ, but its effects are felt throughout the body. The systemic effects of cancer can lead to weakness in muscles and heart, which hastens cancer-associated death. The majority of preclinical studies, including our own, on cancer-induced skeletal muscle defects utilized mouse models and C2C12 myoblast cell line of mouse origin (>7,700 publications). However, a recent study reported distinct metabolic pathways in mouse compared to human. For example, while higher fasting circulating levels of glucose in human is associated with shortened lifespan, it is associated with longer lifespan in mouse. Since the metabolic activity of the skeletal muscle primarily determines circulating glucose levels, skeletal muscle in mouse and human may function differently and not all skeletal muscle defects noted in tumor-bearing mice may not translate into human. Therefore, we developed a robust human model system using human muscle stem cells to understand cancer-induced skeletal muscle defects in human. We found conditioned media from breast cancer cell lines affected cell molecular biomarkers (e.g. PAX7 annd MyOD) and cell surface markers (e.g. CD82, CD54 and CD90) which were associated with dynamic changes of mRNAs, proteins and peptides in human muscle stem cells or human myotubes differentiated from human muscle stem cells. This study will help in cost-effective discovery of drugs that are effective irrespective genetic ancestry and reveal utility of two class of drugs to overcome cancer-induced skeletal muscle defects
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:51.830.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034000
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-19 06:50:50	ID requested
1	2023-05-10 09:06:37	announced
⏵ 2	2023-11-14 08:46:00	announced	2023-11-14: Updated project metadata.

Wang R, Kumar B, Bhat-Nakshatri P, Khatpe AS, Murphy MP, Wanczyk KE, Simpson E, Chen D, Gao H, Liu Y, Doud EH, Mosley AL, Nakshatri H, A human skeletal muscle stem/myotube model reveals multiple signaling targets of cancer secretome in skeletal muscle. iScience, 26(4):106541(2023) [pubmed]

10.6019/PXD034000;

submitter keyword: breast cancer, muscle stem cells, skeletal muscle, LC-MSMS., miR-486, cancer cell line,human, biomarker

Amber Mosley
contact affiliation	IUSM Center for Proteome Analysis, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	Indiana University School of Medicine
contact email	edoud@iu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD034000

PRIDE project URI

• PRIDE
  1. PXD034000
     1. Label: PRIDE project
     2. Name: Mechanisms associated with systemic effects of cancer