PXD033981

PXD033981 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development of parallel reaction monitoring mass spectrometry assay for the detection of human norovirus major capsid protein
Description	Human Norwalk virus (HuNoVs), the most common etiological agents of acute gastroenteritis, are genetically diverse RNA viruses that frequently cause mass food poisoning internationally. In this study, we developed a parallel reaction monitoring (PRM) assay to simultaneously detect and identify representative peptides derived from VP1 in six different HuNoV genotypes using a high-resolution mass spectrometer. An optimized protocol was also used to detect and quantify VP1 protein in human stool specimens. This method is expected to become a new tool for studying the molecular epidemiology of HuNoV and to shed new light on targeted proteomics in clinical practice.
HostingRepository	jPOST
AnnounceDate	2023-02-21
AnnouncementXML	Submission_2023-02-20_21:41:01.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yayoi Kimura
SpeciesList	scientific name: Norwalk virus; NCBI TaxID: 11983; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 21:59:37	ID requested
⏵ 1	2023-02-20 21:41:02	announced

Publication List

Kimura Y, Shin J, Nakai Y, Takahashi M, Ino Y, Akiyama T, Goto K, Nagata N, Yamaoka Y, Miyakawa K, Kimura H, Ryo A, Development of Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of Human Norovirus Major Capsid Protein. Viruses, 14(7):(2022) [pubmed]

Kimura Y, Shin J, Nakai Y, Takahashi M, Ino Y, Akiyama T, Goto K, Nagata N, Yamaoka Y, Miyakawa K, Kimura H, Ryo A, Development of Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of Human Norovirus Major Capsid Protein. Viruses, 14(7):(2022) [pubmed]

Keyword List

submitter keyword: Norwalk virus, Norovirus, Stool specimen, Mass spectrometry, Parallel reaction monitoring (PRM)

submitter keyword: Norwalk virus, Norovirus, Stool specimen, Mass spectrometry, Parallel reaction monitoring (PRM)

Contact List

Akihide Ryo
lab head
Yayoi Kimura
contact affiliation	Yokohama City University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001590/