PXD033979

PXD033979 is an original dataset announced via ProteomeXchange.

Title	COVID-19 PATIENTS WITH NEUROLOGICAL SYMPTOMS PRESENT BROAD CENTRAL NERVOUS SYSTEM MOLECULAR AND STRUCTURAL ALTERATIONS ASSOCIATED WITH SYSTEMIC INFLAMMATION AND DISEASE SEVERITY
Description	Background. COVID-19-induced neurological disease is a growing concern. Here we provide a comprehensive clinical, molecular, and neuroimaging investigation of patients presenting neurological symptoms to investigate underlying processes. Methods. We performed a detailed systematized clinical, laboratory, and neuroimaging (CT/MRI) data analysis from 35 mild to severe Brazilian COVID-19 hospitalised patients with clinical indications for cerebrospinal fluid (CSF) exam. In patients' CSF, we measured interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α) and the Alzheimer's disease-associated biomarkers amyloid-beta (Aβ)1-40, Aβ1-42, Tau and pTau181 levels (n=31-35). In addition, CSF proteomics and the search for SARS-CoV-2 spike protein presence using shotgun and targeted multiple reaction monitoring liquid-chromatography/mass-spectrometry were performed (n=16). We further evaluated a 29-cytokine panel in patients’ blood (n=16). An electronic questionnaire was conducted one-year post-hospitalisation (n=19, 63.3%). Findings. COVID-19 patients presented heterogeneous clinical and neurological features, including encephalopathy, encephalitis, and neuromuscular syndromes, despite low pulmonary burden. Patients showed increased circulating cytokines and CSF IL-6 and TNF-α levels compared to controls. Alzheimer’s disease-related biomarkers were unaltered compared to controls. COVID-19 altered CSF proteomic pathways associated to complement and coagulation cascades, immune-inflammatory, metabolism and amyloidosis. We found no traces of spike protein in patients’ CSF. Severe patients presented more pronounced neuroimaging alterations, altered CSF levels of IL-6, Tau, and Aβ-species compared to mild patients. Peripheral inflammation markers correlated with CSF IL-6 and Tau-species levels. Finally, in a one-year post-COVID survey, survivors were not recovered entirely (12/19) and reported confusion or memory/attention deficits (13/19). Interpretation. COVID-19 induces a broad spectrum of neurological presentations associated with changes in central nervous system (CNS) inflammatory and neurodegenerative molecular and structural biomarkers that correlate with systemic inflammation and disease severity. Given that neurological symptoms persist up to one-year post-COVID, our results urge us to investigate whether systemic, and CNS molecular changes are permanent or alleviated so that COVID-19 patients with lingering symptoms are adequately treated.
HostingRepository	PRIDE
AnnounceDate	2024-08-09
AnnouncementXML	Submission_2024-08-09_06:58:54.867.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033979
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Victor Corasolla Carregari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 07:40:05	ID requested
⏵ 1	2024-08-09 06:58:55	announced

10.1016/j.bbih.2024.100805;

Barros-Arag, ã, o FGQ, Pinto TP, Carregari VC, Rezende NBS, Pinheiro TL, Reis-de-Oliveira G, Cabral-Castro MJ, Queiroz DC, Fonseca PLC, Gon, ç, alves AL, de Freitas GR, Sudo FK, Mattos P, Bozza FA, Rodrigues EC, Aguiar RS, Rodrigues RS, Brand, ã, o CO, Souza AS, Martins-de-Souza D, De Felice FG, Tovar-Moll F, Changes in neuroinflammatory biomarkers correlate with disease severity and neuroimaging alterations in patients with COVID-19 neurological complications. Brain Behav Immun Health, 39():100805(2024) [pubmed]

10.6019/PXD033979;

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: Cerebrospinal fluid, CSF, Inflammation,Prorteomics, COVID-19

Daniel Martins-de-Souza
contact affiliation	Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil
contact email	dmsouza@unicamp.br
lab head
Victor Corasolla Carregari
contact affiliation	Post doctor
contact email	corasolla@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033979
     1. Label: PRIDE project
     2. Name: COVID-19 PATIENTS WITH NEUROLOGICAL SYMPTOMS PRESENT BROAD CENTRAL NERVOUS SYSTEM MOLECULAR AND STRUCTURAL ALTERATIONS ASSOCIATED WITH SYSTEMIC INFLAMMATION AND DISEASE SEVERITY