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PXD033975

PXD033975 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA genetic platform to investigate the functions of bacterial drug efflux pumps
DescriptionEfflux pumps are a significant challenge for the development of new antibacterial agents. Overcoming efflux requires an in-depth understanding of efflux pump functions, substrate specificities, and the development of inhibitors. However, the complexities of drug efflux networks have limited such studies. To address these challenges, we report the generation of Efflux KnockOut-35 (EKO-35), a highly susceptible Escherichia coli strain lacking 35 efflux pumps. We demonstrate the utility of this strain by constructing an efflux platform consisting of strains individually expressing genes encoding efflux pumps forming tripartite complexes with the outer membrane channel TolC. This platform was profiled against a curated diverse compound collection, which enabled us to define physicochemical properties that contribute to transport. We also show the E. coli drug efflux network is conditionally essential for growth, and that the platform can be used to investigate efflux pump inhibitor specificities and also efflux pump interplay. We believe EKO-35 and the efflux platform will have widespread application for the study of drug efflux.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:11.588.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Thompson
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-18 07:32:16ID requested
12022-08-31 07:47:17announced
22023-11-14 08:56:11announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Escherichia coli, Efflux Pumps
Contact List
Georgina Cox
contact affiliationUniversity of Guelph
contact emailgcox@uoguelph.ca
lab head
Laura Thompson
contact affiliationUniversity of Guelph
contact emaillthomp06@uoguelph.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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