PXD033966

PXD033966 is an original dataset announced via ProteomeXchange.

Title	Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein
Description	The nitric oxide synthase interacting protein (NOSIP), an E3-ubiquitin ligase, is involved in various processes like neuronal development, craniofacial development, granulopoiesis, mitogenic signaling, apoptosis and cell proliferation. The best characterized function of NOSIP is the regulation of eNOS (endothelial nitric oxide synthase) activity by translocating the membrane bound enzyme to the actin-skeleton, specifically in the G2 phase of the cell cycle. For this, NOSIP itself has to be translocated from its prominent localization, the nucleus, to the cytoplasm. Nuclear import of NOSIP was suggested to be mediated by the canonical transport receptors importin /. Recently, we found NOSIP in a proteomic screen as a potential importin 13 cargo. Here, we describe the nuclear shuttling characteristics of NOSIP in vivo and in vitro and show that it does not interact directly with importin . Instead, it formed stable complexes with several importins (-, -7, -/7, -5, -13 and transportin 1) and was imported into the nucleus in digitonin-permeabilized cells. In vivo, transportin 1 seems to be the major nuclear transport receptor. A detailed analysis of the NOSIP-transportin 1 interaction revealed an unusual binding mode, involving the N-terminal half of transportin 1. In contrast to nuclear import, nuclear export of NOSIP seems to occur mostly by passive diffusion.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:25:33.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Urlaub
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 07:25:07	ID requested
1	2023-03-10 12:49:01	announced
⏵ 2	2023-11-14 07:25:34	announced	2023-11-14: Updated project metadata.

P, ö, rschke M, Rodr, í, guez-Gonz, á, lez I, Parfentev I, Urlaub H, Kehlenbach RH, Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein. J Biol Chem, 299(3):102932(2023) [pubmed]

submitter keyword: Transportin, nuclear import

Prof. Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Göttingen, Germany Bioanalytics Group, Institute of Clinical Chemistry, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075 Göttingen, Germany
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Henning Urlaub
contact affiliation	Max-Plank Institute for Biophysical Chemistry
contact email	msbio.goettingen@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033966
     1. Label: PRIDE project
     2. Name: Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein