PXD033964

PXD033964 is an original dataset announced via ProteomeXchange.

Title	Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria
Description	Ring-infected erythrocytes are the predominant asexual stage in the peripheral circulation but are rarely investigated in the context of acquired immunity against Plasmodium falciparum malaria. Here we compare antibody-dependent phagocytosis of ring-infected parasite cultures in samples from a controlled human malaria infection (CHMI) study (NCT02739763). Protected volunteers did not develop clinical symptoms, maintained parasitaemia below a predefined threshold of 500 parasites/μl and were not treated until the end of the study. Antibody-dependent phagocytosis of both ring-infected and uninfected erythrocytes from parasite cultures was strongly correlated with protection. A surface proteomic analysis revealed the presence of merozoite proteins including erythrocyte binding antigen-175 and -140 on ring-infected and uninfected erythrocytes, providing an additional antibody-mediated protective mechanism for their activity beyond invasion-inhibition. Competition phagocytosis assays support the hypothesis that merozoite antigens are the key mediators of this functional activity. Targeting ring-stage parasites may contribute to the control of parasitaemia and prevention of clinical malaria.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_05:05:51.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Tuju
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 05:09:38	ID requested
⏵ 1	2022-08-11 05:05:52	announced

Musasia FK, Nkumama IN, Frank R, Kipkemboi V, Schneider M, Mwai K, Odera DO, Rosenkranz M, F, ü, rle K, Kimani D, Tuju J, Njuguna P, Hamaluba M, Kapulu MC, Wardemann H, Osier FHA, Abdi AI, Abebe Y, Bejon P, Billingsley PF, Bull PC, de Laurent Z, Hoffman SL, James ER, Kariuki S, Kimathi R, Kinyanjui S, Kivisi C, Makale J, Marsh K, Mohammed KS, Mosobo M, Musembi J, Musyoki J, Muthui M, Mwacharo J, Ndungu F, Ngoi JM, Ngoto O, Ogutu B, Olewe F, Omuoyo D, Ong'echa J, Otieno E, Shangala J, Sim BKL, Richie TL, Wambua J, Williams TN, Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria. Nat Commun, 13(1):4098(2022) [pubmed]

submitter keyword: Plasmodium, ring stage

Prof Faith Osier
contact affiliation	Centre for Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany
contact email	Fosier@iavi.org
lab head
James Tuju
contact affiliation	KEMRI Wellcome Trust Research Programme
contact email	jtuju@kemri-wellcome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033964

PRIDE project URI

• PRIDE
  1. PXD033964
     1. Label: PRIDE project
     2. Name: Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria