PXD033960

PXD033960 is an original dataset announced via ProteomeXchange.

Title	Confirmation of direct interaction between H3K56cr and GLYR1 by H3K*56cr Site-Link in living cells
Description	As we have found that H3K56cr can crosslink with GLYR1 in living cells, we now perform H3K*cr Site-Link in GLYR1 overexpressed cells. The subsequent 365-nm light irradiation could activate the diazirine and covalently capture potential effector proteins on native chromatin in living cells. The crosslinked band could be identified after gel-based proteomics and pLink assisted MS/MS analysis of the crosslinked peptide.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:41.214.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangfei Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 04:39:46	ID requested
1	2023-03-05 20:31:53	announced
⏵ 2	2023-11-14 08:29:42	announced	2023-11-14: Updated project metadata.

Qin F, Li B, Wang H, Ma S, Li J, Liu S, Kong L, Zheng H, Zhu R, Han Y, Yang M, Li K, Ji X, Chen PR, Linking chromatin acylation mark-defined proteome and genome in living cells. Cell, 186(5):1066-1085.e36(2023) [pubmed]

submitter keyword: pLink, GLYR1, H3K56cr

Peng R. Chen
contact affiliation	Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Executive Editor, ACS Chemical Biology
contact email	pengchen@pku.edu.cn
lab head
Fangfei Qin
contact affiliation	Peking University
contact email	faqpku@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033960

PRIDE project URI

• PRIDE
  1. PXD033960
     1. Label: PRIDE project
     2. Name: Confirmation of direct interaction between H3K56cr and GLYR1 by H3K*56cr Site-Link in living cells