PXD033958

PXD033958 is an original dataset announced via ProteomeXchange.

Title	Life-time study of premodified histone for Identification of chromatinized proteome defined by site-specific histone acylations in living cells
Description	C13, N15 isotope labelled heavy lysine bearing the modification group, such as cr and bhb, were site-sepcifically incorporated into histone H3K56 in living cells for measurement of the modification life of H3K56cr and H3K56bhb without iinterference caused by endogenous histone modification. Utilizaing mass spectrometry, we compared with H3K56cr/bhb peptide with the H3K79 peptide as internal standard at different times. The quantitation analysis of H3K56cr and H3K56bhb at different time points can be comparative.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:42.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangfei Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 04:28:49	ID requested
1	2023-03-05 20:34:48	announced
⏵ 2	2023-11-14 08:30:42	announced	2023-11-14: Updated project metadata.

Qin F, Li B, Wang H, Ma S, Li J, Liu S, Kong L, Zheng H, Zhu R, Han Y, Yang M, Li K, Ji X, Chen PR, Linking chromatin acylation mark-defined proteome and genome in living cells. Cell, 186(5):1066-1085.e36(2023) [pubmed]

submitter keyword: Heavy lysine, H3K56,Life time, bhb, cr

Peng R. Chen
contact affiliation	Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Executive Editor, ACS Chemical Biology
contact email	pengchen@pku.edu.cn
lab head
Fangfei Qin
contact affiliation	Peking University
contact email	faqpku@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033958

PRIDE project URI

• PRIDE
  1. PXD033958
     1. Label: PRIDE project
     2. Name: Life-time study of premodified histone for Identification of chromatinized proteome defined by site-specific histone acylations in living cells