PXD033952

PXD033952 is an original dataset announced via ProteomeXchange.

Title	Proteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells
Description	Here, we develop a new in vitro model replicating the first interaction of FhNEJ with mouse primary small intestinal epithelial cells (MPSIEC). FhNEJ and MPSIEC were incubated for 3 hours and protein extracts (tegument and soma for FhNEJ and membrane and cytosol for MPSIEC) were subjected to quantitative SWATH-MS proteomics compared with respective controls to evaluate the changes in both the parasite and the host.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:08.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Becerro Recio
SpeciesList	scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: 6192;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-18 04:01:19	ID requested
1	2022-09-20 01:24:33	announced
⏵ 2	2023-11-14 08:56:09	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Fasciola hepatica

Newly excysted juveniles

SWATH-MS

Quantitative proteomics

Differential expression

Host-parasite interaction

Mar Siles-Lucas
contact affiliation	Laboratory of Parasitology, Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C/Cordel de Merinas 40-52, 37008 Salamanca, Spain
contact email	mmar.siles@irnasa.csic.es
lab head
David Becerro Recio
contact affiliation	CSIC
contact email	david.becerro@irnasa.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD033952

PRIDE project URI

• PRIDE
  1. PXD033952
     1. Label: PRIDE project
     2. Name: Proteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells