PXD033943

PXD033943 is an original dataset announced via ProteomeXchange.

Title	Proteomics of valve silica deposition vesicles from the diatom Thalassiosira pseudonana
Description	Biomineral forming organisms produce inorganic materials with complex, genetically encoded morphologies that are inaccessible by current synthetic chemistry. It is poorly understood which genes are involved in biomineral morphogenesis and how the encoded proteins guide this process. We addressed these questions using diatoms, which are paradigms for the self-assembly of hierarchically meso- and macroporous silica under mild reaction conditions. By isolating the intracellular organelle for silica biosynthesis, we identified a suite of new biomineralization proteins. Three of these, dAnk1-3, are specific to diatoms and contain a common protein-protein interaction domain indicating a role in coordinating assembly of the silica biomineralization machinery. Knocking out individual dank genes led to characteristic structural aberrations in silica biogenesis that point to a liquid-liquid phase separation process as underlying mechanism for pore pattern morphogenesis. Our work provides an unprecedented path for the synthesis of tailored meso- and macroporous silicas using Synthetic Biology.
HostingRepository	PRIDE
AnnounceDate	2022-11-21
AnnouncementXML	Submission_2022-11-21_08:33:22.975.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033943
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MarcGentzel
SpeciesList	scientific name: Thalassiosira pseudonana CCMP1335; NCBI TaxID: 296543;
ModificationList	methionine oxidation with neutral loss of 64 Da; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-17 08:29:27	ID requested
⏵ 1	2022-11-21 08:33:23	announced

10.6019/PXD033943;

submitter keyword: Silica Deposition Vesicles (SDV),Thalassiosira pseudonana CCMP1335

Prof. Dr. NilsKröger
contact affiliation	B CUBE Technische Universität Dresden Tatzberg 41 03107 Dresden
contact email	nils.kroeger@tu-dresden.de
lab head
MarcGentzel
contact affiliation	Molecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden
contact email	marc.gentzel@tu-dresden.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033943

PRIDE project URI

• PRIDE
  1. PXD033943
     1. Label: PRIDE project
     2. Name: Proteomics of valve silica deposition vesicles from the diatom Thalassiosira pseudonana