PXD033939

PXD033939 is an original dataset announced via ProteomeXchange.

Title	Lysine long-chain fatty acylation regulates the TEAD transcription factor
Description	TEAD transcription factors are responsible for the transcriptional output of Hippo signalling1,2. TEAD activity is primarily regulated by phosphorylation of its coactivators YAP and TAZ3,4. In addition, cysteine palmitoylation has recently been shown to regulate TEAD activity5,6. Here, we report lysine long-chain fatty acylation as a novel posttranslational modification of TEADs. Lysine fatty acylation occurs spontaneously via intramolecular transfer of acyl groups from the proximal acylated cysteine residue. Lysine fatty acylation, like cysteine palmitoylation, contributes to the transcriptional activity of TEADs by enhancing the interaction with YAP and TAZ, but it is more stable than cysteine acylation, suggesting that the lysine fatty-acylated TEAD acts as a “stable active form”. Significantly, lysine fatty acylation of TEAD increased upon Hippo signalling activation, despite a decrease in cysteine acylation. Our results provide new insight into the role of fatty acyl modifications in the regulation of TEAD activity.
HostingRepository	PRIDE
AnnounceDate	2023-03-19
AnnouncementXML	Submission_2023-03-19_16:46:28.413.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033939
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	TakehiroSuzuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	palmitoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-17 08:25:06	ID requested
⏵ 1	2023-03-19 16:46:28	announced

10.6019/PXD033939;

submitter keyword: TEAD acylation

NaoshiDohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
TakehiroSuzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033939

PRIDE project URI

• PRIDE
  1. PXD033939
     1. Label: PRIDE project
     2. Name: Lysine long-chain fatty acylation regulates the TEAD transcription factor