PXD033938

PXD033938 is an original dataset announced via ProteomeXchange.

Title	Proteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice
Description	We obtained the profiles of neuronal proteome after cerebral ischemia and reperfusion by isolating mice hippocampus. Hippocampus combined from either nine sham or nine focal cerebral ischemia 1.5 h and reperfusion 24 h (IR) mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. In total, we quantified 5,059 proteins. We identified 142 differentially expressed proteins (t-test, p-value<0.05) after IR compared to sham groups. The results showed that 92 proteins were upregulated, and 50 proteins were downregulated after IR compared to sham groups. Gene ontology (GO) enrichment analysis of differentially expressed proteins between sham and IR groups. The results showed that the biological process of most of upregulated genes linked with immune inflammatory related responses were increased. And KEGG pathway analysis for upregulated genes showed that multiple immune inflammatory response pathways also increased significantly, such as TNF-signaling, NF-κB signaling and cytokine-cytokine receptor interaction, as well as NOD-like receptor signaling, and toll-like receptor signaling.
HostingRepository	PRIDE
AnnounceDate	2024-02-15
AnnouncementXML	Submission_2024-02-14_20:45:43.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Jiang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-17 08:24:12	ID requested
⏵ 1	2024-02-14 20:45:44	announced

Dataset with its publication pending

submitter keyword: Proteome

hippocampus

cerebral ischemia and reperfusion

Hai Jun Tu
contact affiliation	College of Biology, Hunan University, China
contact email	haijuntu@hnu.edu.cn
lab head
Wei Jiang
contact affiliation	College of Biology, Hunan University
contact email	jiangweibio@hnu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033938
     1. Label: PRIDE project
     2. Name: Proteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice