PXD033916

PXD033916 is an original dataset announced via ProteomeXchange.

Title	Chlorocebus sabaeus vero E6 and and eIF4E(S209A) Vero cells (S209A-Vero) LC-MSMS upon infection by coronavirus PEDV
Description	Two rounds of TMT relative quantitative proteomics were performed to detect cellular factors involved in p-eIF4E regulation of the synthesis of viral proteins.our first round of screening identified differentially expressed proteins in PEDV-infected cells and mock-infected cells; the cellular pathways involved were mainly the estrogen, cAMP, and calcium signaling pathways. Second round screening identified differentially expressed proteins in the PEDV-infected S209A-Vero cells vs. the PEDV-infected WT-Vero cells; the regulated cellular pathways were found to be mainly in the PI3K-Akt, focal adhesion, and mTOR signaling pathways, and the biological processes and molecular functions in which p-eIF4E played a role were related mainly to metabolism and biogenesis, catalytic activity, and stimuli response.4006 host factors were detected, of which 193 (in brown) were significantly upregulated (ratio ≥1.2, P＜0.05) and 191 (in green) were down-regulated upon PEDV infection (ratio ≤0.83, P＜0.05). 29 of the 191 down-regulated proteins were susceptible to a low level of p-eIF4E . Notably, among the 193 upregulated cellular proteins, 77 were upregulated in the WT-Vero over the S209A-Vero cells , suggesting that the WT-Vero cells are more susceptible to a high level of p-eIF4E.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:24:34.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	jing wang
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 08:14:54	ID requested
1	2023-03-10 12:46:16	announced
⏵ 2	2023-11-14 07:24:40	announced	2023-11-14: Updated project metadata.

Dong HJ, Wang J, Zhang XZ, Li CC, Liu JF, Wang XJ, Proteomic screening identifies RPLp2 as a specific regulator for the translation of coronavirus. Int J Biol Macromol, 230():123191(2023) [pubmed]

submitter keyword: Chlorocebus sabaeus vero E6,PEDV, and eIF4E(S209A) Vero cells (S209A-Vero), phosphorylation of eIF4E,LC-MSMS

XiaoJia Wang
contact affiliation	2 Yuanmingyuan West Road, Haidian District, Beijing, P.R. China 100193Yuanmingyuan West Road, Haidian District, Beijing, P.R. China 100193
contact email	wangxj@cau.edu.cn
lab head
jing wang
contact affiliation	school
contact email	2320908036@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033916

PRIDE project URI

• PRIDE
  1. PXD033916
     1. Label: PRIDE project
     2. Name: Chlorocebus sabaeus vero E6 and and eIF4E(S209A) Vero cells (S209A-Vero) LC-MSMS upon infection by coronavirus PEDV