PXD033915

PXD033915 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization and protective effect against brain ischemia produced by adult mouse neural progenitor cells-derived extracellular vesicles.
Description	Hypoxia produced by stroke activates the proliferation of neural progenitor cells (NPC) in the subventricular zone of the adult mouse brain. Newly generated cells seem to participate in the protection of the ischemic brain through the release of extracellular vesicles (EVs) that might target different cell types and modify their function by delivering proteins, lipids, and nucleic acids. We investigated the possible communication route mediated by EVs between NPC and neurons under conditions of cerebral ischemia using an in vitro model of oxygen and glucose deprivation (OGD). We collected OGD-activated NPC-derived EVs by differential centrifugation of the conditioned media in which NPC were grown for 12 h after OGD, and as a control, we harvested EVs released by cells cultured under normoxic conditions. EVs were characterized by shape and size with transmission electron microscopy and NanoTracking assays, and the presence of typical exosome markers was assessed by western blot. The proteomic profiling of NPC-derived EVs was carried out after resolving the proteins by polyacrylamide gel electrophoresis and performed in-gel digestion with trypsin, then the identity of the products was determined by tandem mass spectrometry (ESI-IMS-MS). Overrepresentation analyses of the proteomic data allowed us to identify executioners of signaling pathways involved in neuroprotection. (Supported by DGAPA-PAPIIT IN207020 and CONACYT A1-S-13219).
HostingRepository	PRIDE
AnnounceDate	2023-07-14
AnnouncementXML	Submission_2023-07-13_18:19:44.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EmmanuelRios-Castro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 08:06:15	ID requested
⏵ 1	2023-07-13 18:19:45	announced

Dataset with its publication pending

submitter keyword: stroke,Extracellular vesicles, neural progenitor cells, neurodegeneration

Luis B. Tovar yRomo
contact affiliation	Department of Molecular Neuropathology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico
contact email	ltovar@ifc.unam.mx
lab head
EmmanuelRios-Castro
contact affiliation	Cinvestav-IPN
contact email	eriosc@cinvestav.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033915
     1. Label: PRIDE project
     2. Name: Proteomic characterization and protective effect against brain ischemia produced by adult mouse neural progenitor cells-derived extracellular vesicles.