PXD033900

PXD033900 is an original dataset announced via ProteomeXchange.

Title	Identification of dGTP-interacting mitochondrial proteins by affinity purification and LC-ESI-MS/MS
Description	Imbalanced mitochondrial dNTP pools are known players in the pathogenesis of multiple human diseases. However, we have shown that dGTP is largely overrepresented over the other dNTPs in mitochondria of mouse tissues and human cultured cells. Here, we have performed affinity purification studies and identified NDUFA10, an accessory subunit of respiratory complex I, as the protein binding most of the dGTP contained in mitochondria. This interaction provides a possible link between oxidative metabolism and regulation of dNTP availability and thus with DNA maintenance.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:59.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yolanda Cámara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-16 06:41:44	ID requested
1	2022-06-29 04:39:11	announced
⏵ 2	2023-11-14 08:57:00	announced	2023-11-14: Updated project metadata.

Molina-Granada D, Gonz, á, lez-Vioque E, Dibley MG, Cabrera-P, é, rez R, Vallbona-Garcia A, Torres-Torronteras J, Sazanov LA, Ryan MT, C, á, mara Y, Mart, í R, Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit. Commun Biol, 5(1):620(2022) [pubmed]

submitter keyword: Mitochondria,Mouse, pull-down, dGTP

Ramon Martí
contact affiliation	Research Group on Neuromuscular and Mitochondrial Disorders, Vall d’Hebron Institut de Recerca, Universitat Autònoma de Barcelona, Barcelona, Catalonia
contact email	ramon.marti@vhir.org
lab head
Yolanda Cámara
contact affiliation	Vall d'Hebron Research Institute-CIBERER
contact email	yolanda.camara@vhir.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD033900

PRIDE project URI

• PRIDE
  1. PXD033900
     1. Label: PRIDE project
     2. Name: Identification of dGTP-interacting mitochondrial proteins by affinity purification and LC-ESI-MS/MS