PXD033889

PXD033889 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of KIT-containing EVs secreted by neoplastic human mast cells (SUB1)
Description	Activating mutations in the receptor KIT promote the dysregulated proliferation of human mast cells (huMCs). The resulting neoplastic huMCs secrete extracellular vesicles (EVs) that can transfer oncogenic KIT among other cargo into recipient cells. Despite potential contributions to diseases, KIT-containing EVs have not been thoroughly investigated. Here, we isolated KIT-EV subpopulations released by neoplastic huMCs using an immunocapture approach that selectively isolates EVs containing KIT in its proper topology. Proteomic profiling was performed on KIT-containing EVs compared to KIT-depleted EV populations, and on KIT-EVs that are microvesicle- and exosome-like.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:16.616.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033889
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Anderson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-13 06:50:53	ID requested
1	2023-03-10 10:59:16	announced
⏵ 2	2023-11-14 08:28:19	announced	2023-11-14: Updated project metadata.

Pfeiffer A, Petersen JD, Falduto GH, Anderson DE, Zimmerberg J, Metcalfe DD, Olivera A, Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesicles. J Extracell Vesicles, 11(10):e12272(2022) [pubmed]

10.6019/PXD033889;

submitter keyword: exosomes, microvesicles, extracellular vesicles,KIT, immunocapture, mast cell

Dean Darrel Metcalfe
contact affiliation	Mast Cell Biology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectios Diseases, National Istitutes of Health, Department of Health and Human Services, United States Government.
contact email	dmetcalfe@niaid.nih.gov
lab head
David Anderson
contact affiliation	NIH/NIDDK
contact email	davida@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD033889

PRIDE project URI

• PRIDE
  1. PXD033889
     1. Label: PRIDE project
     2. Name: Proteomic profiling of KIT-containing EVs secreted by neoplastic human mast cells (SUB1)