PXD033886

PXD033886 is an original dataset announced via ProteomeXchange.

Title	Overexpression of McHB7 Transcription Factor from Mesembryanthemum crystallinum Improves Plant Salt Tolerance
Description	Mesembryanthemum crystallinum (common ice plant) is one of the facultative halophyte plants, and it serves as a model for investigating the molecular mechanisms underlying its salt stress response and tolerance. Here we cloned one of homeobox transcription factor (TF) gene McHB7 from ice plant, which has 60% similarity with the Arabidopsis AtHB7. Overexpression of McHB7 in Arabidopsis (OE) showed that the plants had significantly elevated relative water content (RWC), chlorophyll content, superoxide dismutase (SOD) and peroxidase (POD) activities after salt stress treatment. Proteomics analysis identified 145 to be significantly changed in abundance, and 66 were exclusively increased in the OE plants compared to wild type (WT). After salt treatment, 979 and 959 metabolites were significantly increased and decreased in OE plants compared to the WT, respectively. The results demonstrated McHB7 can improve photosynthesis and increase the leaf chlorophyll content, and affect TCA cycle by regulating metabolites (e.g., pyruvate) and proteins (e.g., citrate synthase). Also, McHB7 modulates the expression of stress-related proteins (e.g., superoxide dismutase, dehydroascorbate reductase and pyrroline-5-carboxylate synthase B) to scavenge reactive oxygen species and enhance plant salt tolerance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:49.733.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bowen Tan
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-13 06:49:44	ID requested
1	2022-08-11 05:30:44	announced
⏵ 2	2024-10-22 05:38:54	announced	2024-10-22: Updated project metadata.

10.3390/ijms23147879;

Zhang X, Tan B, Cheng Z, Zhu D, Jiang T, Chen S, Improves Plant Salt Tolerance. Int J Mol Sci, 23(14):(2022) [pubmed]

submitter keyword: Salt stress;common ice plant;proteomics

Sixue Chen
contact affiliation	Department of Biology, Genetics Institute, UF interdisciplinary Center for Biotechnology Research, Plant Molecular and Cellular Biology Program, University of Florida
contact email	schen@ufl.edu
lab head
Bowen Tan
contact affiliation	PhD student
contact email	tanbowen@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033886

PRIDE project URI

• PRIDE
  1. PXD033886
     1. Label: PRIDE project
     2. Name: Overexpression of McHB7 Transcription Factor from Mesembryanthemum crystallinum Improves Plant Salt Tolerance