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PXD033875

PXD033875 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes
DescriptionIn agroecosystems, a plant-usable form of nitrogen is mainly generated by legume-based biological nitrogen fixation, a process that requires phosphorus (P) as an essential nutrient. To investigate the physiological mechanism whereby phosphorus influences soybean nodule nitrogen fixation, soybean root nodules were exposed to four phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress), 31 mg/L (Normal P), 61 mg/L (High P) then proteome analysis of nodules was conducted to identify phosphorus-associated proteome changes. We found that phosphorus stress-induced ribosomal protein structural changes were associated with altered key root nodule protein synthesis profiles. Importantly, up-regulated expression of peroxidase was observed as an important phosphorus stress-induced nitrogen fixation-associated adaptation that supported two nodule-associated activities: scavenging of reactive oxygen species (ROS) and cell wall growth. In addition, phosphorus transporter (PT) and purple acid phosphatase (PAPs) were up-regulated that regulated phosphorus transport and utilisation to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules.
HostingRepositoryPRIDE
AnnounceDate2022-08-11
AnnouncementXMLSubmission_2022-08-11_04:10:48.443.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYubo Yao
SpeciesList scientific name: Vigna mungo (Rice bean) (Black gram); NCBI TaxID: 3915;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive HF; Q-Tof Ultima; 6410 Triple Quadrupole LC/MS; Orbitrap Fusion; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-12 10:10:35ID requested
12022-08-11 04:10:49announced
Publication List
Yao Y, Yuan H, Wu G, Ma C, Gong Z, Proteome Analysis of the Soybean Nodule Phosphorus Response Mechanism and Characterization of Stress-Induced Ribosome Structural and Protein Expression Changes. Front Plant Sci, 13():908889(2022) [pubmed]
Keyword List
submitter keyword: Proteome, Soybean, Phosphorus, Root nodule, Nitrogen fixation
Contact List
Yubo Yao
contact affiliationInstitute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
contact emailyaoyubo2009@aliyun.com
lab head
Yubo Yao
contact affiliationHeilongjiang Academy of Agricultural Sciences
contact emailyaoyubo2009@aliyun.com
dataset submitter
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Dataset FTP location
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