PXD033875 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes |
Description | In agroecosystems, a plant-usable form of nitrogen is mainly generated by legume-based biological nitrogen fixation, a process that requires phosphorus (P) as an essential nutrient. To investigate the physiological mechanism whereby phosphorus influences soybean nodule nitrogen fixation, soybean root nodules were exposed to four phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress), 31 mg/L (Normal P), 61 mg/L (High P) then proteome analysis of nodules was conducted to identify phosphorus-associated proteome changes. We found that phosphorus stress-induced ribosomal protein structural changes were associated with altered key root nodule protein synthesis profiles. Importantly, up-regulated expression of peroxidase was observed as an important phosphorus stress-induced nitrogen fixation-associated adaptation that supported two nodule-associated activities: scavenging of reactive oxygen species (ROS) and cell wall growth. In addition, phosphorus transporter (PT) and purple acid phosphatase (PAPs) were up-regulated that regulated phosphorus transport and utilisation to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-11 |
AnnouncementXML | Submission_2022-08-11_04:10:48.443.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yubo Yao |
SpeciesList | scientific name: Vigna mungo (Rice bean) (Black gram); NCBI TaxID: 3915; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive HF; Q-Tof Ultima; 6410 Triple Quadrupole LC/MS; Orbitrap Fusion; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 10:10:35 | ID requested | |
⏵ 1 | 2022-08-11 04:10:49 | announced | |
Publication List
Yao Y, Yuan H, Wu G, Ma C, Gong Z, Proteome Analysis of the Soybean Nodule Phosphorus Response Mechanism and Characterization of Stress-Induced Ribosome Structural and Protein Expression Changes. Front Plant Sci, 13():908889(2022) [pubmed] |
Keyword List
submitter keyword: Proteome, Soybean, Phosphorus, Root nodule, Nitrogen fixation |
Contact List
Yubo Yao |
contact affiliation | Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
contact email | yaoyubo2009@aliyun.com |
lab head | |
Yubo Yao |
contact affiliation | Heilongjiang Academy of Agricultural Sciences |
contact email | yaoyubo2009@aliyun.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033875
- Label: PRIDE project
- Name: Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes