PXD033875

PXD033875 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes
Description	In agroecosystems, a plant-usable form of nitrogen is mainly generated by legume-based biological nitrogen fixation, a process that requires phosphorus (P) as an essential nutrient. To investigate the physiological mechanism whereby phosphorus influences soybean nodule nitrogen fixation, soybean root nodules were exposed to four phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress), 31 mg/L (Normal P), 61 mg/L (High P) then proteome analysis of nodules was conducted to identify phosphorus-associated proteome changes. We found that phosphorus stress-induced ribosomal protein structural changes were associated with altered key root nodule protein synthesis profiles. Importantly, up-regulated expression of peroxidase was observed as an important phosphorus stress-induced nitrogen fixation-associated adaptation that supported two nodule-associated activities: scavenging of reactive oxygen species (ROS) and cell wall growth. In addition, phosphorus transporter (PT) and purple acid phosphatase (PAPs) were up-regulated that regulated phosphorus transport and utilisation to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_04:10:48.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yubo Yao
SpeciesList	scientific name: Vigna mungo (Rice bean) (Black gram); NCBI TaxID: 3915;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF; Q-Tof Ultima; 6410 Triple Quadrupole LC/MS; Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 10:10:35	ID requested
⏵ 1	2022-08-11 04:10:49	announced

Yao Y, Yuan H, Wu G, Ma C, Gong Z, Proteome Analysis of the Soybean Nodule Phosphorus Response Mechanism and Characterization of Stress-Induced Ribosome Structural and Protein Expression Changes. Front Plant Sci, 13():908889(2022) [pubmed]

submitter keyword: Proteome, Soybean, Phosphorus, Root nodule, Nitrogen fixation

Yubo Yao
contact affiliation	Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
contact email	yaoyubo2009@aliyun.com
lab head
Yubo Yao
contact affiliation	Heilongjiang Academy of Agricultural Sciences
contact email	yaoyubo2009@aliyun.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033875

PRIDE project URI

• PRIDE
  1. PXD033875
     1. Label: PRIDE project
     2. Name: Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes