PXD033872

PXD033872 is an original dataset announced via ProteomeXchange.

Title	The selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis
Description	Thioredoxin/glutathione reductase (TGR, TXNRD3) is a selenoprotein composed of thioredoxin reductase and glutaredoxin domains; the function of this enzyme remains unknown. This NADPH-dependent thiol oxidoreductase evolved by gene duplication within the Txnrd family, is expressed in the testes and can reduce both thioredoxin and glutathione in vitro. To characterize the function of TXNRD3 in vivo, we generated a strain of mice with the deletion of Txnrd3 gene. We show that Txnrd3 knockout mice are viable and without discernable gross phenotypes, but TXNRD3 deficiency leads to fertility impairment in male mice. Txnrd3 knockout animals exhibit a lower fertilization rate in vitro, a sperm movement phenotype and an altered redox status of thiols. Proteomic analyses revealed a broad range of substrates reduced by TXNRD3 during sperm maturation, presumably as a part of quality control. The results show that TXNRD3 plays a critical role in male reproduction via the thiol redox control of spermatogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:06:26.943.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 09:55:39	ID requested
1	2022-10-13 02:45:14	announced
⏵ 2	2023-11-14 07:06:27	announced	2023-11-14: Updated project metadata.

Dou Q, Turanov AA, Mariotti M, Hwang JY, Wang H, Lee SG, Paulo JA, Yim SH, Gygi SP, Chung JJ, Gladyshev VN, Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis. J Biol Chem, 298(8):102183(2022) [pubmed]

submitter keyword: redox control, sperm motility,Thioredoxin glutathione reductase, male fertility

Vadim Gladyshev
contact affiliation	Harvard Institutes of Medicine, BWH/Genetics 77 Avenue Louis Pasteur, Boston MA 02115, United States
contact email	vgladyshev@rics.bwh.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033872
     1. Label: PRIDE project
     2. Name: The selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis