PXD033859

PXD033859 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based analysis of LRBA phosphorylation
Description	Vasopressin/cAMP/protein kinase A (PKA) signaling phosphorylates AQP2 water channels in renal collecting ducts to reabsorb water from urine for the prevention of further water loss. Lipopolysaccharide-responsive and beige-like anchor protein (LRBA) mediates vasopressin-induced AQP2 phosphorylation; therefore LRBA is essential for urinary concentration. LRBA is identified as the PKA substrates in a mouse cortical collecting duct principal cell line (mpkCCDcl4) whose phosphorylation levels are nearly perfectly correlated with those of AQP2. Although mouse LRBA contains several consensus PKA phosphorylation sites, their phosphorylation status in response to vasopressin remain unknown. Post-translational modification analysis revealed that RRDS1607 and RRIS2189 were phosphorylated by vasopressin.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:24.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YU HARA
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 08:59:09	ID requested
1	2022-07-22 20:43:12	announced
⏵ 2	2023-11-14 08:56:24	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LRBA, PKA, vasopressin

Shinichi Uchida
contact affiliation	Department of Nephrology, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
contact email	suchida.kid@tmd.ac.jp
lab head
YU HARA
contact affiliation	Department of Nephrology, Tokyo Medical and Dental University (TMDU)
contact email	yhara.kid@tmd.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD033859

PRIDE project URI

• PRIDE
  1. PXD033859
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based analysis of LRBA phosphorylation