PXD033856

PXD033856 is an original dataset announced via ProteomeXchange.

Title	Disturbance of key cellular subproteomes upon propofol treatment is associated with increased permeability of the blood-brain barrier
Description	Background: Propofol is a short-acting anesthetic, which is often used for induction and maintenance of general anesthesia, sedation for mechanically ventilated adults and procedural sedation. Several side effects of propofol are known and a substantial number of patients suffer from post-operative delirium after propofol application. In this study, we analyzed the effect of propofol on the function and protein expression profile on a proteome-wide scale. Methods: We cultured human brain microvascular endothelial cells in the absence and presence of propofol and analyzed the permeability of the blood-brain barrier (BBB) by fluorescein passage and protein expression on a proteome-wide scale by mass spectrometry. Results: Propofol interfered with the function of the blood-brain barrier. This was not due to de-creased adhesion of propofol-treated human brain microvascular endothelial cells. The proteomic analysis revealed that some key pathways in these cells were disturbed, such as oxygen metabolism, DNA damage recognition and response to stress. Conclusions: Propofol has strong effects on protein expression which could explain several side effects of propofol.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:01:34.364.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033856
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matt Fuszard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 08:53:26	ID requested
1	2022-12-02 09:17:29	announced
⏵ 2	2023-11-14 08:01:35	announced	2023-11-14: Updated project metadata.

L, ä, ngrich T, Bork K, Horstkorte R, Weber V, Hofmann B, Fuszard M, Olzscha H, Disturbance of Key Cellular Subproteomes upon Propofol Treatment Is Associated with Increased Permeability of the Blood-Brain Barrier. Proteomes, 10(3):(2022) [pubmed]

10.6019/PXD033856;

submitter keyword: anesthetics

blood-brain barrier

DNA damage response

drug effect

human brain microvascular endothelial cells

metabolic stress

propofol

proteome

quantitative proteomics

reactive oxygen species (ROS)

Heidi Olzscha
contact affiliation	Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale); Germany
contact email	Heidi.Olzscha@medizin.uni-halle.de
lab head
Matt Fuszard
contact affiliation	Martin-Luther-Üniversität Halle-Wittenberg
contact email	matthew.fuszard@medizin.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033856
     1. Label: PRIDE project
     2. Name: Disturbance of key cellular subproteomes upon propofol treatment is associated with increased permeability of the blood-brain barrier