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PXD033852

PXD033852 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlecAMP/PKA signaling phosphorylates LRBA in renal collecting duct
DescriptionVasopressin/cAMP/protein kinase A (PKA)/aquaporin-2 (AQP2) water channels signaling in the kidneys is a canonical pathway that determines AQP2 activity in response to body fluid balance. AQP2 phosphorylation by PKA increases water reabsorption from urine for the prevention of further water loss. We used several activators of cAMP/PKA signaling as screening tools to generate various phosphorylation patterns of PKA substrates and AQP2 in a mouse cortical collecting duct principal cell line (mpkCCDcl4). We next quantified phosphorylation levels of PKA substrates and AQP2 after western blot analysis using a phospho-PKA substrates antibody. Finally, we screen for PKA substrates whose phosphorylation levels were well correlated with those of AQP2. Immunoprecipitation with a phospho-PKA substrates antibody followed by mass spectrometry analysis revealed that the leading candidate in this assay proved to be a lipopolysaccharide-responsive and beige-like anchor protein (LRBA). Phosphorylation levels of LRBA was nearly perfectly correlated with those of AQP2.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:58.682.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYU HARA
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-12 08:48:11ID requested
12022-07-22 20:44:52announced
22023-11-14 08:56:59announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PKA substrates, AQP2, LRBA, renal collecting duct
Contact List
Shinichi Uchida
contact affiliationDepartment of Nephrology, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
contact emailsuchida.kid@tmd.ac.jp
lab head
YU HARA
contact affiliationDepartment of Nephrology, Tokyo Medical and Dental University (TMDU)
contact emailyhara.kid@tmd.ac.jp
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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