PXD033852 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | cAMP/PKA signaling phosphorylates LRBA in renal collecting duct |
Description | Vasopressin/cAMP/protein kinase A (PKA)/aquaporin-2 (AQP2) water channels signaling in the kidneys is a canonical pathway that determines AQP2 activity in response to body fluid balance. AQP2 phosphorylation by PKA increases water reabsorption from urine for the prevention of further water loss. We used several activators of cAMP/PKA signaling as screening tools to generate various phosphorylation patterns of PKA substrates and AQP2 in a mouse cortical collecting duct principal cell line (mpkCCDcl4). We next quantified phosphorylation levels of PKA substrates and AQP2 after western blot analysis using a phospho-PKA substrates antibody. Finally, we screen for PKA substrates whose phosphorylation levels were well correlated with those of AQP2. Immunoprecipitation with a phospho-PKA substrates antibody followed by mass spectrometry analysis revealed that the leading candidate in this assay proved to be a lipopolysaccharide-responsive and beige-like anchor protein (LRBA). Phosphorylation levels of LRBA was nearly perfectly correlated with those of AQP2. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:58.682.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | YU HARA |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 08:48:11 | ID requested | |
1 | 2022-07-22 20:44:52 | announced | |
⏵ 2 | 2023-11-14 08:56:59 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: PKA substrates, AQP2, LRBA, renal collecting duct |
Contact List
Shinichi Uchida |
contact affiliation | Department of Nephrology, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan |
contact email | suchida.kid@tmd.ac.jp |
lab head | |
YU HARA |
contact affiliation | Department of Nephrology, Tokyo Medical and Dental University (TMDU) |
contact email | yhara.kid@tmd.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033852
- Label: PRIDE project
- Name: cAMP/PKA signaling phosphorylates LRBA in renal collecting duct