PXD033852

PXD033852 is an original dataset announced via ProteomeXchange.

Title	cAMP/PKA signaling phosphorylates LRBA in renal collecting duct
Description	Vasopressin/cAMP/protein kinase A (PKA)/aquaporin-2 (AQP2) water channels signaling in the kidneys is a canonical pathway that determines AQP2 activity in response to body fluid balance. AQP2 phosphorylation by PKA increases water reabsorption from urine for the prevention of further water loss. We used several activators of cAMP/PKA signaling as screening tools to generate various phosphorylation patterns of PKA substrates and AQP2 in a mouse cortical collecting duct principal cell line (mpkCCDcl4). We next quantified phosphorylation levels of PKA substrates and AQP2 after western blot analysis using a phospho-PKA substrates antibody. Finally, we screen for PKA substrates whose phosphorylation levels were well correlated with those of AQP2. Immunoprecipitation with a phospho-PKA substrates antibody followed by mass spectrometry analysis revealed that the leading candidate in this assay proved to be a lipopolysaccharide-responsive and beige-like anchor protein (LRBA). Phosphorylation levels of LRBA was nearly perfectly correlated with those of AQP2.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:58.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YU HARA
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 08:48:11	ID requested
1	2022-07-22 20:44:52	announced
⏵ 2	2023-11-14 08:56:59	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: PKA substrates, AQP2, LRBA, renal collecting duct

Shinichi Uchida
contact affiliation	Department of Nephrology, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
contact email	suchida.kid@tmd.ac.jp
lab head
YU HARA
contact affiliation	Department of Nephrology, Tokyo Medical and Dental University (TMDU)
contact email	yhara.kid@tmd.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD033852

PRIDE project URI

• PRIDE
  1. PXD033852
     1. Label: PRIDE project
     2. Name: cAMP/PKA signaling phosphorylates LRBA in renal collecting duct