PXD033847

PXD033847 is an original dataset announced via ProteomeXchange.

Title	Loss of ERG in human lung endothelial cells leads to release of pro-inflammatory and pro-fibrotic factors.
Description	: Although vascular dysfunction is a hallmark of chronic aging-associated diseases, including idiopathic pulmonary fibrosis, the role of the pulmonary vasculature to lung repair versus lung fibrosis is not fully understood. We identified the endothelial transcription factor ETS-related gene (ERG) as an orchestrator of vascular homeostasis and repair following lung injury. To evaluate whether loss of endothelial ERG influences the activation of neighboring naïve lung fibroblasts, we collected the conditioned media (CM) generated by control- and ERG-silenced human lung endothelial cells (ECs) and we applied them to normal human lung fibroblasts. We found that CM from ERG-silenced human lung ECs strongly promoted human lung fibroblast activation and enhanced the effect of the fibrogenic mediator TGF. In support of these results, analysis of CM using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) revealed increased secretion of numerous pro-inflammatory and pro-fibrogenic mediators by ERG-silenced human lung ECs in comparison to control-silenced human lung ECs.
HostingRepository	PRIDE
AnnounceDate	2022-06-24
AnnouncementXML	Submission_2022-06-24_06:39:29.726.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033847
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Madden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 04:31:41	ID requested
⏵ 1	2022-06-24 06:39:30	announced

Dataset with its publication pending

submitter keyword: lung, vasculature, fibrosis, ERG

Nunzia Caporarello
contact affiliation	Mayo Clinic, Assistant Professor of Physiology
contact email	Caporarello.Nunzia@mayo.edu
lab head
Benjamin Madden
contact affiliation	Mayo Clinic
contact email	madden.benjamin@mayo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033847
     1. Label: PRIDE project
     2. Name: Loss of ERG in human lung endothelial cells leads to release of pro-inflammatory and pro-fibrotic factors.