PXD033846

PXD033846 is an original dataset announced via ProteomeXchange.

Title	APEX2 proximity labeling of PI(3)P dependent ER and early endosome membrane contact tether proteins
Description	To understand the nature of ER and early endosome membrane contact site, we capitalized the MTM1 knock out (KO) HeLa cells that do not dissociate the ER and early endosome membrane contact upon nutrient starvation. By using the Doxycyclin inducible APEX2 fused Rab5A (early endosome marker) expressing stable cell lines of wildtype (WT) or KO cells, early endsome proximity proteins were acutely biotinyated during starvation. Subsequent biochemical fractionation were conducted to enrich ER membrane and biotinylated proteins were furhter enriched for Mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:28:35.300.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wonyul Jang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 04:31:10	ID requested
1	2023-01-05 23:45:30	announced
⏵ 2	2023-11-14 07:28:36	announced	2023-11-14: Updated project metadata.

Jang W, Puchkov D, Sams, ó P, Liang Y, Nadler-Holly M, Sigrist SJ, Kintscher U, Liu F, Mamchaoui K, Mouly V, Haucke V, Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function. Science, 378(6625):eabq5209(2022) [pubmed]

submitter keyword: HeLa, APEX2, LC-MS, MTM1, early endosome, ER, membrane contact

Volker Haucke
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact email	Haucke@fmp-berlin.de
lab head
Wonyul Jang
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact email	jang@fmp-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD033846

PRIDE project URI

• PRIDE
  1. PXD033846
     1. Label: PRIDE project
     2. Name: APEX2 proximity labeling of PI(3)P dependent ER and early endosome membrane contact tether proteins