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PXD033833

PXD033833 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCatechol-O-methyl transferase suppresses cell invasion and interplays with MET signaling in estrogen dependent breast cancer
DescriptionCatechol-O-methyl transferase (COMT) is involved in detoxification of catechol estrogens, playing cancer-protective role in cells producing or utilizing estrogen. Moreover, COMT suppressed migration potential of breast cancer cells. To delineate COMT role in metastasis of estrogen receptor dependent BC, we investigated the effect of COMT overexpression on invasion, transcriptome, proteome and interactome of MCF7 cells, a luminal A breast cancer model, stably transduced with lentiviral vector carrying COMT gene (MCF7-COMT). This PRIDE project includes quantitative analysis results for the total proteome LC-DIA-MS/MS experiment evaluating COMT overexpression in MCF7 breast cancer cell line, and results of pulldown analysis of COMT-interacting proteins in MCF7 cells.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:29.387.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPavel Bouchal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-12 02:24:01ID requested
12023-01-31 10:02:20announced
22023-11-14 08:56:29announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: pulldown, breast cancer, COMT, proteomics,DIA
Contact List
Pavel Bouchal
contact affiliationMasaryk University, Faculty of Science
contact emailbouchal@chemi.muni.cz
lab head
Pavel Bouchal
contact affiliationMasaryk University, Faculty of Science
contact emailbouchal@chemi.muni.cz
dataset submitter
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Dataset FTP location
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PRIDE project URI
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