PXD033831

PXD033831 is an original dataset announced via ProteomeXchange.

Title	Employing Sterculic Acid–a naturally occurring 1, 2-cyclopropene fatty acid–for live-cell imaging of lipids by inverse electron-demand Diels-Alder reaction
Description	To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene containing oleic acid analogue, as a bioorthogonal probe. We here show that this lipid can be readily taken up by dendritic cells without toxic side-effects, and that it can subsequently be visualised in live cells using an inverse electron-demand Diels-Alder (IEDDA) reaction with quenched tetrazine fluorophores. Furthermore, this reaction can be integrated into a multiplexed bioorthogonal reaction workflow by combining it with two sequential copper-catalysed Huisgen ligation reactions. This allows for the study of multiple biomolecules in the cell simultaneously by multimodal confocal imaging. Aside from lipid imaging, the uptake and protein modification of sterculic acid can be studied in live cells via chemical proteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:17.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Berend Gagestein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 02:23:17	ID requested
1	2023-04-28 01:33:41	announced
⏵ 2	2023-11-14 08:45:21	announced	2023-11-14: Updated project metadata.

Bertheussen K, van de Plassche M, Bakkum T, Gagestein B, Ttofi I, Sarris AJC, Overkleeft HS, van der Stelt M, van Kasteren SI, Live-Cell Imaging of Sterculic Acid-a Naturally Occurring 1,2-Cyclopropene Fatty Acid-by Bioorthogonal Reaction with Turn-On Tetrazine-Fluorophore Conjugates. Angew Chem Int Ed Engl, 61(38):e202207640(2022) [pubmed]

submitter keyword: lipidation, PTM,Bioorthogonal, sterculic, cyclopropene

Mario van der Stelt
contact affiliation	Professor of Molecular physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Berend Gagestein
contact affiliation	Leiden University
contact email	b.gagestein@lic.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD033831

PRIDE project URI

• PRIDE
  1. PXD033831
     1. Label: PRIDE project
     2. Name: Employing Sterculic Acid–a naturally occurring 1, 2-cyclopropene fatty acid–for live-cell imaging of lipids by inverse electron-demand Diels-Alder reaction