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PXD033821

PXD033821 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative Proteomics Analysis of the PEG Stress Response in a Sorghum Variety and Its Mutant
DescriptionDrought stress severely limits plant growth and development, however, the molecular and genetic basis of sorghum (Sorghum bicolor (L.) Moench) response to drought stress is poorly understood. To unravel the molecular mechanism of drought tolerance of sorghum, comparative proteomics analysis was performed on drought-tolerant variety JTY and its EMS drought-sensitive mutant E24 seedlings treated with 25% PEG 6000 simulated drought stress for 24 hours. Over 3000 proteins were accurately quantified from four samples. KEGG analysis showed that after PEG treatment, the pathways related to carbohydrate metabolism were affected, Starch synthase was decreased, while plant-pathogen interaction and phenylpropanoid biosynthesis were significantly enhanced under PEG stress. Ribosome, Biosynthesis of amino acids, Carbon metabolism and Photosynthesis were common enriched by mutant related DAPs and mutant response to PEG stress. some stress and mutant related DAPs were verified by parallel reaction monitoring (PRM) and qRT-PCR, respectively. Our findings offer insights into the molecular networks mediating sorghum response to drought stress.
HostingRepositoryiProX
AnnounceDate2022-05-12
AnnouncementXMLSubmission_2022-05-11_20:30:22.350.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaoping Wang
SpeciesList scientific name: Sorghum bicolor x Saccharum hybrid cultivar; NCBI TaxID: 1325292;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-11 20:30:05ID requested
12022-05-11 20:30:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomics, Drought response, EMS Mutant, Sorghum
Contact List
Yinghong Pan
contact affiliationChinese Academy of Agricultural Sciences
contact emailpanyinghong@caas.cn
lab head
Daoping Wang
contact affiliationChinese Academy of Agricultural Sciences
contact emailwangdaoping_2016@163.com
dataset submitter
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iProX dataset URI