PXD033818

PXD033818 is an original dataset announced via ProteomeXchange.

Title	Edwardsiella tarda strain EIB 202 lysine acetylation and succinylation
Description	The antibiotics resistance of Edwardsiella tarda is prevailing, seeking a novel antimicrobial strategies extremely urgent. Lysine acylation has been proved to play important role on the bacterial physiological function while its role on bacterial antibiotics resistance mechanism still largely unclear. In this study, we investigated the lysine acetylation and succinylation profiles of E. tarda stain EIB202 using affinity antibody purification combined with LC MS/MS. A total of 1511 lysine-acetylation sites were identified on 589 proteins, and 2346 lysine-succinylation sites were further identified on 692 proteins of this pathogen. Further bioinformatics analysis showed that both PTMs prefer to enrich in TCA cycle, pyruvate metabolism. biosynthesis, and carbon metabolism. Besides, 948 peptides of 437 proteins were found to be overlap and associated with multiple metabolic pathways. Moreover, both the acetylation and succinylation were found in many antimicrobial resistance (AMR) proteins, suggesting its potentially vital role in antibiotic resistance. In general, our work provides insights into the acetylome and succinylome features responsible for the antibiotic resistance mechanism of E. tarda, which may facilitate future investigations on the pathogenesis of this bacterium.
HostingRepository	iProX
AnnounceDate	2022-05-12
AnnouncementXML	Submission_2022-09-28_19:44:57.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuying Fu
SpeciesList	scientific name: Edwardsiella tarda EIB202; NCBI TaxID: 498217;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-11 20:22:30	ID requested
1	2022-05-11 20:22:45	announced
⏵ 2	2022-09-28 19:44:57	announced	2022-09-29: Update publication information.

Fu Y, Zhang L, Song H, Liao J, Lin L, Jiang W, Wu X, Wang G, Reveals Key Roles of Both Lysine Acylations in Bacterial Antibiotic Resistance. Antibiotics (Basel), 11(7):(2022) [pubmed]

submitter keyword: Edwardsiella tarda, acetylome, succinylome, antibiotics resistance

Xiangmin Lin
contact affiliation	Fujian Agriculture and Forestry University
contact email	313461278@qq.com
lab head
Yuying Fu
contact affiliation	Fujian Agriculture and Forestry University
contact email	773842814@qq.com
dataset submitter

iProX dataset URI