PXD033814

PXD033814 is an original dataset announced via ProteomeXchange.

Title	Influence of a high dietary level of the microalgae Chlorella vulgaris alone and combined with exogenous carbohydrases on muscle transcriptome and proteome of finishing pigs: an integrated approach
Description	Background: monogastric feeding is dependent on conventional feedstuffs such as corn and soybean meal. However, these are increasingly expensive and environmentally unsustainable. Therefore, there is an increasing interest in high quality and sustainable alternative feedstuffs, namely microalgae. Chlorella vulgaris is one of such alternatives, with a high level of crude protein and bioactive compounds. However, its recalcitrant cell wall hinders the digestive process. CAZyme supplementation is a possible solution to overcome such a problem. The objective of this work was to evaluate the effect of a 5% dietary C. vulgaris (CV) and enzymatic supplementation (CV+R – Rovabio® Excel AP; CV+M – four CAZyme mix) on muscle transcriptome and proteome of finishing pigs in an integrated approach. Results: there were no differences concerning the live pig production performance. Pigs that were fed with the microalga had increased deposition of n-3 PUFA, including C20:5n-3 (EPA) in the muscle. Control pigs increased the abundance of contractile apparatus (e.g. MYH1, MYH2, MYH4) and energy metabolism (e.g. CKMT1, NDUFS3) proteins, demonstrating increased nutrient availability. Moreover, they had increased abundance of SCD, characteristic of increased glucose availability via the activation of SREBP-1c and ChREBP. CV and CV+R pigs upregulated proteolytic and apoptotic genes (e.g. BAX, DDA1), whilst increasing the abundance of glucose (UQCRFS1) and fatty acid catabolism (ACADS) proteins. CV+R pigs upregulated ACOT8 and SIRT3 genes as a response to reduced nutrient availability, to maintain energy homeostasis and oxidative status. The CV+M pigs showed the least number of significant differences in both omics platforms. Conclusions: the four-CAZyme mix designed to degrade the microalgal cell wall was efficient in re-establishing the muscle metabolism, mitigating the otherwise negative effects of microalga incorporation in the diet.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:05.384.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Miguel Ribeiro
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-11 06:29:53	ID requested
1	2023-03-11 05:48:15	announced
⏵ 2	2023-11-14 08:43:09	announced	2023-11-14: Updated project metadata.

Coelho D, Ribeiro D, Os, ó, rio H, de Almeida AM, Prates JAM, Integrated Omics analysis of pig muscle metabolism under the effects of dietary Chlorella vulgaris and exogenous enzymes. Sci Rep, 12(1):16992(2022) [pubmed]

submitter keyword: CAZymes, muscle proteome,Chlorella vulgaris, antioxidant potential, muscle transcriptome, finishing pigs, fatty acid metabolism

José António Mestre Prates
contact affiliation	CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Alto da Ajuda, 1300-477 Lisboa, Portugal.
contact email	japrates@fmv.ulisboa.pt
lab head
David Miguel Ribeiro
contact affiliation	LEAF Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, University of Lisbon, Lisboa, Portugal
contact email	davidribeiro@isa.ulisboa.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033814
     1. Label: PRIDE project
     2. Name: Influence of a high dietary level of the microalgae Chlorella vulgaris alone and combined with exogenous carbohydrases on muscle transcriptome and proteome of finishing pigs: an integrated approach