PXD033812

PXD033812 is an original dataset announced via ProteomeXchange.

Title	The proteome data of human mesenchymal stem cell derived from healthy donors
Description	The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detected by bicinchoninic acid assay (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Inc., Sunnyvale, CA, USA). Protein digestion was carried out by a filter-aided sample preparation technique. Briefly, 50 μg of a sample was loaded onto a Microcon filter unit YM-30 (Millipore) and washed with 8 M urea, followed by protein alkylation with 50 mM iodoacetamide in 8 M urea in darkness for 20 min. After alkylation, the filters were washed twice with 8 M urea and twice with 40 mM ammonium bicarbonate. Trypsin (Promega, Madison, WI, USA) was added to the samples at 100:1 protein-to-enzyme ratio and incubated overnight at 37 °C. Later the same
HostingRepository	iProX
AnnounceDate	2023-06-12
AnnouncementXML	Submission_2023-08-14_00:32:52.338.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchen Gao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-11 06:05:58	ID requested
1	2022-05-11 06:13:07	announced
⏵ 2	2023-08-14 00:32:53	announced	2023-08-14: Update publication information.

Gao Y, Chi Y, Chen Y, Wang W, Li H, Zheng W, Zhu P, An J, Duan Y, Sun T, Liu X, Xue F, Liu W, Fu R, Han Z, Zhang Y, Yang R, Cheng T, Wei J, Zhang L, Zhang X, Multi-omics analysis of human mesenchymal stem cells shows cell aging that alters immunomodulatory activity through the downregulation of PD-L1. Nat Commun, 14(1):4373(2023) [pubmed]

submitter keyword: mesenchymal stem cell, senescence

Lei Zhang
contact affiliation	State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	zhanglei1@ihcams.ac.cn
lab head
Yuchen Gao
contact affiliation	State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
contact email	821120396@qq.com
dataset submitter

iProX dataset URI